Effects of human amnion-derived mesenchymal stromal cell transplantation in rats with radiation proctitis

Ono, Masayoshi; Ohnishi, Shunsuke; Honda, Minori; Ishikawa, Marin; Hosono, Hidetaka; Onishi, Reizo; Nakagawa, Koji; Takeda, Hiroshi; Sakamoto, Naoya

Effects of human amnion-derived mesenchymal stromal cell transplantation in rats with radiation proctitis

Ono, Masayoshi; Ohnishi, Shunsuke; Honda, Minori et al.

Cytotherapy, 2015, 17(11), 1545-1559

Permalink : https://hdl.handle.net/2115/63375

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アクセス権	open access
URI	https://hdl.handle.net/2115/63375
タイトル	Effects of human amnion-derived mesenchymal stromal cell transplantation in rats with radiation proctitis
著者	Ono, Masayoshi ; Ohnishi, Shunsuke ; Honda, Minori ; Ishikawa, Marin ; Hosono, Hidetaka ; Onishi, Reizo ; Nakagawa, Koji ; Takeda, Hiroshi ; Sakamoto, Naoya
著者名 Ono, Masayoshi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Honda, Minori
著者名 Ishikawa, Marin
著者名 Hosono, Hidetaka
著者名 Onishi, Reizo
e-Rad_Researcher 80360949 著者名 Nakagawa, Koji
e-Rad_Researcher 60261294 著者名 Takeda, Hiroshi
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	amnion
	cell injury
	mesenchymal stromal cells
	radiation proctitis
発行日	2015-11
出版者	Elsevier
収録物名	Cytotherapy
巻(号)	17(11)
ページ	1545-1559
抄録	Background aims. Mesenchymal stromal cells (MSCs) have been reported to be a promising cell source in cell therapy, and large amounts of MSCs can easily be isolated from human amnion. Therapeutic irradiation for intra-pelvic cancer often causes radiation proctitis; however, there is currently no effective treatment. We therefore investigated the effect of transplantation of human amnion derived MSCs (AMSCs) in rats with radiation proctitis. Methods. Amnion was obtained at cesarean delivery, and AMSCs were isolated and expanded. Sprague-Dawley rats were gamma-irradiated (5 Gy/d) at the rectum for 5 days. On day 5, AMSCs (1 x 10(6) cells) were intravenously transplanted. Rats were killed on day 8. Histological analyses were performed, and messenger RNA expression of inflammatory mediators was measured. In vitro, after gamma-irradiation of rat intestinal epithelial cells (IEC-6), the cells were cultured with AMSC-conditioned medium (CM). The effect of AMSC-CM was evaluated by measurement of caspase-3/7 activity, p53 transcription activity and quantitative reverse-transcription polymerase chain reaction for p53-target genes. Results. Histological examination demonstrated that epithelial injury and infiltration of inflammatory cells in the rectum were significantly suppressed by transplantation of AMSCs. In vitro, the cell injury in IEC-6 cells induced by gamma-irradiation was inhibited by AMSC-CM, which also inhibited the upregulation of p53 transcription activity, caspase-3/7 activity and p21 expression. Conclusions. Transplantation of AMSCs improved radiation proctitis, possibly through inhibition of cell injury and inflammatory reactions. AMSC transplantation should be considered as a new treatment for radiation proctitis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1465-3249
関連情報	PMID 26256683
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jcyt.2015.07.003