Carbon nanohorns allow acceleration of osteoblast differentiation via macrophage activation

Hirata, Eri; Miyako, Eijiro; Hanagata, Nobutaka; Ushijima, Natsumi; Sakaguchi, Norihito; Russier, Julie; Yudasaka, Masako; Iijima, Sumio; Bianco, Alberto; Yokoyama, Atsuro

Carbon nanohorns allow acceleration of osteoblast differentiation via macrophage activation

Hirata, Eri; Miyako, Eijiro; Hanagata, Nobutaka et al.

Nanoscale, 2016, 8(30), 14514-14522

Permalink : https://hdl.handle.net/2115/63383

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アクセス権	open access
URI	https://hdl.handle.net/2115/63383
タイトル	Carbon nanohorns allow acceleration of osteoblast differentiation via macrophage activation
著者	Hirata, Eri ; Miyako, Eijiro ; Hanagata, Nobutaka ; Ushijima, Natsumi ; Sakaguchi, Norihito ; Russier, Julie ; Yudasaka, Masako ; Iijima, Sumio ; Bianco, Alberto ; Yokoyama, Atsuro
著者名 Hirata, Eri
著者名 Miyako, Eijiro
e-Rad_Researcher 10302796 著者名 Hanagata, Nobutaka
著者名 Ushijima, Natsumi
e-Rad_Researcher 70344489 著者名 Sakaguchi, Norihito
著者名 Russier, Julie
e-Rad_Researcher 70159226 著者名 Yudasaka, Masako
著者名 Iijima, Sumio
著者名 Bianco, Alberto
e-Rad_Researcher 20210627 著者名 Yokoyama, Atsuro
言語	英語
発行日	2016-08
出版者	Royal Society of Chemistry
収録物名	Nanoscale
巻(号)	8(30)
ページ	14514-14522
抄録	Carbon nanohorns (CNHs), formed by a rolled graphene structure and terminating in a cone, are promising nanomaterials for the development of a variety of biological applications. Here we demonstrate that alkaline phosphatase activity is dramatically increased by coculture of human monocyte derived macrophages (hMDMs) and human mesenchymal stem cells (hMSCs) in the presence of CNHs. CNHs were mainly localized in the lysosome of macrophages more than in hMSCs during coculturing. At the same time, the amount of Oncostatin M (OSM) in the supernatant was also increased during incubation with CNHs. Oncostatin M (OSM) from activated macrophage has been reported to induce osteoblast differentiation and matrix mineralization through STAT3. These results suggest that the macrophages engulfed CNHs and accelerated the differentiation of mesenchymal stem cells into the osteoblast via OSM release. We expect that the proof-of-concept on the osteoblast differentiation capacity by CNHs will allow future studies focused on CNHs as ideal therapeutic materials for bone regeneration.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 3.0 Unported
PISSN	2040-3364
EISSN	2040-3372
関連情報	PMID 27412794
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1039/c6nr02756c