Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp C34 and Alcaligenes faecalis T1

Sun, Jian; Matsumoto, Ken'ichiro; Tabata, Yuta; Kadoya, Ryosuke; Ooi, Toshihiko; Abe, Hideki; Taguchi, Seiichi

Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp C34 and Alcaligenes faecalis T1

Sun, Jian; Matsumoto, Ken'ichiro; Tabata, Yuta et al.

Applied microbiology and biotechnology, 2015, 99(22), 9555-9563

Permalink : https://hdl.handle.net/2115/63400

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/63400
タイトル	Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp C34 and Alcaligenes faecalis T1
著者	Sun, Jian ; Matsumoto, Ken'ichiro ; Tabata, Yuta ; Kadoya, Ryosuke ; Ooi, Toshihiko ; Abe, Hideki ; Taguchi, Seiichi
著者名 Sun, Jian
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
著者名 Tabata, Yuta
著者名 Kadoya, Ryosuke
e-Rad_Researcher 40223713 著者名 Ooi, Toshihiko
著者名 Abe, Hideki
e-Rad_Researcher 70216828 著者名 Taguchi, Seiichi
言語	英語
キーワード	Biobased polyester
	Polylactic acid
	Biodegradation
	Biodegradable material
	Size-dependent hydrolysis
	Monomer sequence specificity
発行日	2015-11
出版者	Springer
収録物名	Applied microbiology and biotechnology
巻(号)	99(22)
ページ	9555-9563
抄録	Polyhydroxyalkanoate depolymerase derived from Variovorax sp. C34 (PhaZ(Vs)) was identified as the first enzyme that is capable of degrading isotactic P[67 mol% (R)-lactate(LA)-co-(R)-3-hydroxybutyrate(3HB)] [P(d-LA-co-d-3HB)]. This study aimed at analyzing the monomer sequence specificity of PhaZ(Vs) for hydrolyzing P(LA-co-3HB) in comparison with a P(3HB) depolymerase from Alcaligenes faecalis T1 (PhaZ(Af)) that did not degrade the same copolymer. Degradation of P(LA-co-3HB) by action of PhaZ(Vs) generated dimers, 3HB-3HB, 3HB-LA, LA-3HB, and LA-LA, and the monomers, suggesting that PhaZ(Vs) cleaved the linkages between LA and 3HB units and between LA units. To provide a direct evidence for the hydrolysis of these sequences, the synthetic methyl trimers, 3HB-3HB-3HB, LA-LA-3HB, LA-3HB-LA, and 3HB-LA-LA, were treated with the PhaZs. Unexpectedly, not only PhaZ(Vs) but also PhaZ(Af) hydrolyzed all of these substrates, namely PhaZ(Af) also cleaved LA-LA linkage. Considering the fact that both PhaZs did not degrade P[(R)-LA] (PDLA) homopolymer, the cleavage capability of LA-LA linkage by PhaZs was supposed to depend on the length of the LA-clustering region in the polymer chain. To test this hypothesis, PDLA oligomers (6 to 40 mer) were subjected to the PhaZ assay, revealing that there was an inverse relationship between molecular weight of the substrates and their hydrolysis efficiency. Moreover, PhaZ(Vs) exhibited the degrading activity toward significantly longer PDLA oligomers compared to PhaZ(Af). Therefore, the cleaving capability of PhaZs used here toward the d-LA-based polymers containing the LA-clustering region was strongly associated with the substrate length, rather than the monomer sequence specificity of the enzyme.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0175-7598
関連情報	PMID 26109003
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00253-015-6756-1