Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro

Wada, Junya; Miyazaki, Hiromitsu; Kamada, Rui et al.

Chemistry Letters, 2016, 45(2), 185-187

Permalink : https://hdl.handle.net/2115/63555

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ファイル	フォーマット	サイズ	閲覧回数	説明
CL45-2 185-187	pdf	843 KB	439
cl.151019_1	pdf	230 KB	433	Supporting Information

アクセス権	open access
URI	https://hdl.handle.net/2115/63555
タイトル	Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro
著者	Wada, Junya ; Miyazaki, Hiromitsu ; Kamada, Rui ; Sakaguchi, Kazuyasu
著者名 Wada, Junya
著者名 Miyazaki, Hiromitsu
著者名 Kamada, Rui
e-Rad_Researcher 00315053 著者名 Sakaguchi, Kazuyasu
言語	英語
発行日	2016-02
出版者	Chemical Society of Japan
収録物名	Chemistry Letters
巻(号)	45(2)
ページ	185-187
抄録	Protein expression using Escherichia coli is a common and important method for recombinant protein production. Herein, we quantitatively analyzed the correlation between protein expression in vivo and thermodynamic structure stability in vitro using the tetramerization domain of tumor suppressor protein p53. We found a strong positive relationship between the expression level and the thermodynamic stability. Our study suggests that a minimum thermodynamic stability of a protein is required for substantial protein expression in bacterial cells.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0366-7022
EISSN	1348-0715
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1246/cl.151019