Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution

Onodera, Yasuyuki; Arakawa, Takumi; Yui-Kurino, Rika; Yamamoto, Masayuki P; Kitazaki, Kazuyoshi; Ebe, Shigehiko; Matsunaga, Muneyuki; Taguchi, Kazunori; Kuroda, Yosuke; Yamashita, Shiko; Sakai, Tomoyuki; Kinoshita, Toshiro; Mikami, Tetsuo; Kubo, Tomohiko

Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution

Onodera, Yasuyuki; Arakawa, Takumi; Yui-Kurino, Rika et al.

Euphytica, 2015, 206(2), 365-379

Permalink : https://hdl.handle.net/2115/63730

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アクセス権	open access
URI	https://hdl.handle.net/2115/63730
タイトル	Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution
著者	Onodera, Yasuyuki ; Arakawa, Takumi ; Yui-Kurino, Rika ; Yamamoto, Masayuki P ; Kitazaki, Kazuyoshi ; Ebe, Shigehiko ; Matsunaga, Muneyuki ; Taguchi, Kazunori ; Kuroda, Yosuke ; Yamashita, Shiko ; Sakai, Tomoyuki ; Kinoshita, Toshiro ; Mikami, Tetsuo ; Kubo, Tomohiko
著者名 Onodera, Yasuyuki
著者名 Arakawa, Takumi
著者名 Yui-Kurino, Rika
著者名 Yamamoto, Masayuki P
著者名 Kitazaki, Kazuyoshi
著者名 Ebe, Shigehiko
著者名 Matsunaga, Muneyuki
著者名 Taguchi, Kazunori
著者名 Kuroda, Yosuke
著者名 Yamashita, Shiko
著者名 Sakai, Tomoyuki
e-Rad_Researcher 10001421 著者名 Kinoshita, Toshiro
e-Rad_Researcher 50133715 著者名 Mikami, Tetsuo
e-Rad_Researcher 40261333 著者名 Kubo, Tomohiko
言語	英語
キーワード	F1 hybrid
	mitochondrial evolution
	mitochondrial gene
	nuclear-cytoplasmic interaction
	plant mitochondria
	substoichiometric DNA
発行日	2015-11
出版者	Springer
収録物名	Euphytica
巻(号)	206(2)
ページ	365-379
抄録	I-12CMS(2) and I-12CMS(3) are sugar beet lines with different sources of cytoplasmic male sterility (CMS) derived from wild beets in Turkey and Pakistan, respectively. We established that I-12CMS(2) has a genetically distinct cytoplasm, but its mitochondrial genome is very similar to I-12CMS(3). Male fertility was assessed in F1 hybrids produced with a common pollen parent. Fertility in the F1's carrying the I-12CMS(3) cytoplasm exceeded that of the F1's with the I-12CMS(2) cytoplasm. Organization of the I-12CMS(2) and I-12CMS(3) mitochondrial genomes were compared based on their physical maps. Mitochondrial genomes of the two strains were largely collinear, except for a large deletion in the noncoding region of I-12CMS(2). Because a mitochondrial orf129 in the I-12CMS(3) cytoplasm is associated with a male sterility phenotype and preservation of orf129 was evident in I-12CMS(2), I-12CMS(2) orf129 was investigated in detail. I-12CMS(2) plants contained three to five times more ORF129 protein than did I-12CMS(3) plants. A single nucleotide substitution, present in the putative promoter region of orf129, appeared to be responsible for the differential accumulation of orf129 transcript. A long N-terminal extension of atp6 is a common feature of some beet CMSs and is found in I-12CMS(2), but the amino acid sequence is unique. I-12CMS(3) mitochondria, but not I-12CMS(2) mitochondria, were found to be heteroplasmic. This heteroplasmy is characterized by a substoichiometric DNA molecule(s) that has at least two I-12CMS(2)-type mitochondrial loci, suggesting the possibility that the I-12CMS(2) mitochondrial genome might have evolved from such a substoichiometric DNA molecule in I-12CMS(3) mitochondria.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0014-2336
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10681-015-1484-2