Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation

Fukumoto, Tatsuya; Matsuo, Junji; Okubo, Torahiko; Nakamura, Shinji; Miyamoto, Kentaro; Oka, Kentaro; Takahashi, Motomichi; Akizawa, Kouji; Shibuya, Hitoshi; Shimizu, Chikara; Yamaguchi, Hiroyuki

Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation

Fukumoto, Tatsuya; Matsuo, Junji; Okubo, Torahiko et al.

BMC Microbiology, 2016, 16(1), 292

Permalink : https://hdl.handle.net/2115/63951

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アクセス権	open access
URI	https://hdl.handle.net/2115/63951
タイトル	Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation
著者	Fukumoto, Tatsuya ; Matsuo, Junji ; Okubo, Torahiko ; Nakamura, Shinji ; Miyamoto, Kentaro ; Oka, Kentaro ; Takahashi, Motomichi ; Akizawa, Kouji ; Shibuya, Hitoshi ; Shimizu, Chikara ; Yamaguchi, Hiroyuki
著者名 Fukumoto, Tatsuya
e-Rad_Researcher 50359486 著者名 Matsuo, Junji
著者名 Okubo, Torahiko
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
著者名 Miyamoto, Kentaro
著者名 Oka, Kentaro
著者名 Takahashi, Motomichi
著者名 Akizawa, Kouji
著者名 Shibuya, Hitoshi
e-Rad_Researcher 00292029 著者名 Shimizu, Chikara
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	Acanthamoeba
	Environmental chlamydiae
	Hospital
	IL-8
	Genome sequence
発行日	2016-12-15
出版者	BioMed Central
収録物名	BMC Microbiology
巻(号)	16(1)
ページ	292
抄録	Background: Environmental chlamydiae belonging to the Parachlamydiaceae are obligate intracellular bacteria that infect Acanthamoeba, a free-living amoeba, and are a risk for hospital-acquired pneumonia. However, whether amoebae harboring environmental chlamydiae actually survive in hospital environments is unknown. We therefore isolated living amoebae with symbiotic chlamydiae from hospital environments. Results: One hundred smear samples were collected from Hokkaido University Hospital, Sapporo, Japan; 50 in winter (February to March, 2012) and 50 in summer (August, 2012), and used for the study. Acanthamoebae were isolated from the smear samples, and endosymbiotic chlamydial traits were assessed by infectivity, cytokine induction, and draft genomic analysis. From these, 23 amoebae were enriched on agar plates spread with heatkilled Escherichia coli. Amoeba prevalence was greater in the summer-collected samples (15/30, 50%) than those of the winter season (8/30, 26.7%), possibly indicating a seasonal variation (p = 0.096). Morphological assessment of cysts revealed 21 amoebae (21/23, 91%) to be Acanthamoeba, and cultures in PYG medium were established for 11 of these amoebae. Three amoebae contained environmental chlamydiae; however, only one amoeba (Acanthamoeba T4) with an environmental chlamydia (Protochlamydia W-9) was shown the infectious ability to Acanthamoeba C3 (reference amoebae). While Protochlamydia W-9 could infect C3 amoeba, it failed to replicate in immortal human epithelial, although exposure of HEp-2 cells to living bacteria induced the proinflammatory cytokine, IL-8. Comparative genome analysis with KEGG revealed similar genomic features compared with other Protochlamydia genomes (UWE25 and R18), except for a lack of genes encoding the type IV secretion system. Interestingly, resistance genes associated with several antibiotics and toxic compounds were dentified. Conclusion: These findings are the first demonstration of the distribution in a hospital of a living Acanthamoeba carrying an endosymbiotic chlamydial pathogen.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
EISSN	1471-2180
NCID	AA12035256
関連情報	PMID 27978822
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s12866-016-0906-1