Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger

Liao, Julan; Okuyama, Masayuki; Ishihara, Keigo; Yamori, Yoshinori; Iki, Shigeo; Tagami, Takayoshi; Mori, Haruhide; Chiba, Seiya; Kimura, Atsuo

Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger

Liao, Julan; Okuyama, Masayuki; Ishihara, Keigo et al.

Bioscience biotechnology and biochemistry, 2016, 80(9), 1747-1752

Permalink : https://hdl.handle.net/2115/64479

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アクセス権	open access
URI	https://hdl.handle.net/2115/64479
タイトル	Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger
著者	Liao, Julan ; Okuyama, Masayuki ; Ishihara, Keigo ; Yamori, Yoshinori ; Iki, Shigeo ; Tagami, Takayoshi ; Mori, Haruhide ; Chiba, Seiya ; Kimura, Atsuo
著者名 Liao, Julan
e-Rad_Researcher 00344490 著者名 Okuyama, Masayuki
著者名 Ishihara, Keigo
著者名 Yamori, Yoshinori
著者名 Iki, Shigeo
著者名 Tagami, Takayoshi
e-Rad_Researcher 80241363 著者名 Mori, Haruhide
e-Rad_Researcher 30001449 著者名 Chiba, Seiya
e-Rad_Researcher 90186312 著者名 Kimura, Atsuo
言語	英語
キーワード	glycoside hydrolase family 27
	α-galactosidase
	substrate inhibition
	substrate specificity
発行日	2016-09
出版者	Taylor & Francis
収録物名	Bioscience biotechnology and biochemistry
巻(号)	80(9)
ページ	1747-1752
抄録	The recombinant AglB produced by Pichia pastoris exhibited substrate inhibition behavior for the hydrolysis of p-nitrophenyl -galactoside, whereas it hydrolyzed the natural substrates, including galactomanno-oligosaccharides and raffinose family oligosaccharides, according to the Michaelian kinetics. These contrasting kinetic behaviors can be attributed to the difference in the dissociation constant of second substrate from the enzyme and/or to the ability of the leaving group of the substrates. The enzyme displays the grater k(cat)/K-m values for hydrolysis of the branched -galactoside in galactomanno-oligosaccharides than that of raffinose and stachyose. A sequence comparison suggested that AglB had a shallow active-site pocket, and it can allow to hydrolyze the branched -galactosides, but not linear raffinose family oligosaccharides.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 09 Feb 2016, available online: http://wwww.tandfonline.com/10.1080/09168451.2015.1136884.
PISSN	0916-8451
関連情報	PMID 26856407
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1080/09168451.2015.1136884