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https://hdl.handle.net/2115/64525

Effects of Pretreatments on Cryopreservation in Asparagus Shoot Apices

日本語

Ultrastructural changes

1995-12-28

低温生物工学会

低温生物工学会誌

41(2)

50-57

Shoot apices of asparagus (Asparagus officinalis L.) can be successfully cryopreserved (by freezing at a rate of 0.5℃／min in the presence of 8% DMSO and 0.2 M sorbitol) after pretreat­ ments either by preculture in media containing 0.5 M glucose for 2 days or by cold acclimation at O℃ for 20 days. In the present study, we examined the effects of pretreatments for subsequent successful cryopreservation of the shoot apices. Freezing tolerance, as revealed by LT50 under equilibrium-freezing, was 20℃ in both specimens with pretreatment by 0.5 M glucose and by cold acclimation, while LT50 was -7℃ in control specimens. Both pretreatments produced distinct cellular ultrastructural changes as observed with ultra-thin section electron microscopy. Number of plastids rich in starch granules increased in precultured specimens, whereas those poor in the starch granules increased in cold acclimated specimens. Occurrence of freezing injury by equilibrium-freezing, as observed with a freeze-fracture replica technique, was associated with formation of aparticulate domains with accompanying fracture-jump lesions in the plasma membranes, not only in precultured specimens with 0.5 M glucose and in cold acclimated specimens, but also in control specimens.

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1340-7902

AN10448734