Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host

Fujii, Ryuya; Ugai, Takahiro; Ichinose, Hirofumi; Hatakeyama, Mayumi; Kosaki, Takuto; Gomi, Katsuya; Fujii, Isao; Minami, Atsushi; Oikawa, Hideaki

Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host

Fujii, Ryuya; Ugai, Takahiro; Ichinose, Hirofumi et al.

Bioscience biotechnology and biochemistry, 2016, 80(3), 426-431

Permalink : https://hdl.handle.net/2115/64655

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アクセス権	open access
URI	https://hdl.handle.net/2115/64655
タイトル	Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host
著者	Fujii, Ryuya ; Ugai, Takahiro ; Ichinose, Hirofumi ; Hatakeyama, Mayumi ; Kosaki, Takuto ; Gomi, Katsuya ; Fujii, Isao ; Minami, Atsushi ; Oikawa, Hideaki
著者名 Fujii, Ryuya
著者名 Ugai, Takahiro
著者名 Ichinose, Hirofumi
著者名 Hatakeyama, Mayumi
著者名 Kosaki, Takuto
著者名 Gomi, Katsuya
著者名 Fujii, Isao
著者名 Minami, Atsushi
e-Rad_Researcher 00185175 著者名 Oikawa, Hideaki
言語	英語
キーワード	biosynthesis
	heterologous expression
	Aspergillus oryzae
	solanapyrone
	cytochalasin
発行日	2016-03-04
出版者	Taylor & Francis
収録物名	Bioscience biotechnology and biochemistry
巻(号)	80(3)
ページ	426-431
抄録	Reconstitution of whole biosynthetic genes in Aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. Heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. In the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic genes. In this paper, we reported structure determination of by-products and described a simple solution to avoid the undesired reaction by introducing the downstream gene in the heterologous production of solanapyrone C.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 2016.3.4, available online: http://wwww.tandfonline.com/DOI:10.1080/09168451.2015.1104234.
PISSN	0916-8451
関連情報	PMID 26539980
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1080/09168451.2015.1104234