Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Nagatomo, Hiroaki; Kohri, Nanami; Akizawa, Hiroki et al.

Animal science journal, 2016, 87(3), 457-461

Permalink : https://hdl.handle.net/2115/64658

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/64658
タイトル
Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development
著者
Nagatomo, Hiroaki ; Kohri, Nanami ; Akizawa, Hiroki ; Hoshino, Yumi ; Yamauchi, Nobuhiko ; Kono, Tomohiro ; Takahashi, Masashi ; Kawahara, Manabu
著者名
Nagatomo, Hiroaki
著者名
Kohri, Nanami
著者名
Akizawa, Hiroki
著者名
Hoshino, Yumi
著者名
Yamauchi, Nobuhiko
著者名
Kono, Tomohiro
e-Rad_Researcher
10343964
著者名
Takahashi, Masashi
e-Rad_Researcher
70468700
著者名
Kawahara, Manabu
言語
英語
キーワード
bovine embryo
knockdown
NASP
発行日
2016-03
出版者
Wiley-Blackwell
収録物名
Animal science journal
巻(号)
87(3)
ページ
457-461
抄録
Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This is the peer reviewed version of the following article:Animal Science Journal, Volume 87(3),pages 457–461, March 2016, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/asj.12538/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN
1344-3941
関連情報
PMID
26690724
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1111/asj.12538