open access

https://hdl.handle.net/2115/64932

Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl

英語

1999-06

Wiley-Blackwell

Physiologia Plantarum

106(2)

224-231

A cDNA clone of an auxin up-regulated gene, ARG8, was isolated from hypocotyl sections of etiolated mung bean [Vigna radiata (L.) Wilczek] seedlings by differential screening. The deduced amino acid sequence suggested that ARG8 may encode a cell wall protein. The steady state mRNA level of ARG8 increased by treatment of hypocotyl sections not only with indole-3-acetic acid (IAA) but also with fusicoccin, and the auxin inducibility was inhibited by the addition of 0.3 M mannitol in the incubation medium. This indicated that it was not auxin but elongation that regulated the expression of ARG8. The promoter activity of the 5′-flanking region of ARG8 was determined by assaying the transient expression of a luciferase fusion gene that was introduced into mung bean hypocotyl sections by the particle bombardment technique. The basal activity of the ARG8 upstream region was about a few tenths of that of a modified cauliflower mosaic virus 35S promoter, and it was increased a few fold by treatment with IAA. The auxin inducibility was completely suppressed by the addition of mannitol. A 5′-deletion analysis showed that a 53-bp region in the ARG8 promoter was important for the basal and elongation-dependent promoter activities.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl, which has been published in final form at [10.1034/j.1399-3054.1999.106212.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

0031-9317

1399-3054

AA00774354