Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation

Miyata, Saori; Miyaji, Hirofumi; Kawasaki, Hideya; Yamamoto, Masaki; Nishida, Erika; Takita, Hiroko; Akasaka, Tsukasa; Ushijima, Natsumi; Iwanaga, Toshihiko; Sugaya, Tsutomu

Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation

Miyata, Saori; Miyaji, Hirofumi; Kawasaki, Hideya et al.

International Journal of Nanomedicine, 2017, 12, 2703-2716

Permalink : https://hdl.handle.net/2115/65029

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アクセス権	open access
URI	https://hdl.handle.net/2115/65029
タイトル	Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation
著者	Miyata, Saori ; Miyaji, Hirofumi ; Kawasaki, Hideya ; Yamamoto, Masaki ; Nishida, Erika ; Takita, Hiroko ; Akasaka, Tsukasa ; Ushijima, Natsumi ; Iwanaga, Toshihiko ; Sugaya, Tsutomu
著者名 Miyata, Saori
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
著者名 Kawasaki, Hideya
著者名 Yamamoto, Masaki
著者名 Nishida, Erika
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
著者名 Ushijima, Natsumi
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
e-Rad_Researcher 10211301 著者名 Sugaya, Tsutomu
言語	英語
キーワード	Aggregatibacter actinomycetemcomitans
	antimicrobial photodynamic therapy
	photosensitizer
	Porphyromonas gingivalis
	singlet oxygen
	Streptococcus mutans
発行日	2017-04-04
出版者	Dove Medical Press
収録物名	International Journal of Nanomedicine
巻(号)	12
ページ	2703-2716
抄録	Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1O2 generation of Au25(Capt)18 clusters under blue LED irradiation (420–460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1O2 generation ability of Au25(Capt)18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au25(Capt)18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1O2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 3.0 Unported
PISSN	1176-9114
EISSN	1178-2013
NCID	AA1215861X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2147/IJN.S131602