open access

https://hdl.handle.net/2115/65152

Nonylphenol diethoxylate inhibits apoptosis induced in PC12 cells

英語

PC12 cells

2016

Wiley

Environmental Toxicology

31(11)

1389-1398

Nonylphenol and short-chain nonylphenol ethoxylates such as NP2EO are present in aquatic environment as wastewater contaminants, and their toxic effects on aquatic species have been reported. Apoptosis has been shown to be induced by serum deprivation or copper treatment. To understand the toxicity of nonylphenol diethoxylate, we investigated the effects of NP2EO on apoptosis induced by serum deprivation and copper by using PC12 cell system. Nonylphenol diethoxylate itself showed no toxicity and recovered cell viability from apoptosis. In addition, nonylphenol diethoxylate decreased DNA fragmentation caused by apoptosis in PC12 cells. This phenomenon was confirmed after treating apoptotic PC12 cells with nonylphenol diethoxylate, whereas the cytochrome c release into the cytosol decreased compared to that in apoptotic cells not treated with nonylphenol diethoxylates. Furthermore, Bax contents in apoptotic cells were reduced after exposure to nonylphenol diethoxylate. Thus, nonylphenol diethoxylate has the opposite effect on apoptosis in PC12 cells compared to nonylphenol, which enhances apoptosis induced by serum deprivation. The difference in structure of the two compounds is hypothesized to be responsible for this phenomenon. These results indicated that nonylphenol diethoxylate has capability to affect cell differentiation and development and has potentially harmful effect on organisms because of its unexpected impact on apoptosis.

学術雑誌論文

Submitted Manuscript Under Review

This is the peer reviewed version of the following article: http://onlinelibrary.wiley.com/doi/10.1002/tox.22144/epdf, which has been published in final form at 10.1002/tox.22144. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

1520-4081