Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana

Masuda, Seiji; Nozawa, Kosuke; Matsunaga, Wataru; Masuta, Yukari; Kawabe, Akira; Kato, Atsushi; Ito, Hidetaka

Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana

Masuda, Seiji; Nozawa, Kosuke; Matsunaga, Wataru et al.

Genes & Genetic Systems, 2016, 91(6), 293-299

Permalink : https://hdl.handle.net/2115/65346

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/65346
タイトル	Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana
著者	Masuda, Seiji ; Nozawa, Kosuke ; Matsunaga, Wataru ; Masuta, Yukari ; Kawabe, Akira ; Kato, Atsushi ; Ito, Hidetaka
著者名 Masuda, Seiji
著者名 Nozawa, Kosuke
著者名 Matsunaga, Wataru
著者名 Masuta, Yukari
著者名 Kawabe, Akira
e-Rad_Researcher 90177428 著者名 Kato, Atsushi
e-Rad_Researcher 70582295 著者名 Ito, Hidetaka
言語	英語
キーワード	Arabidopsis
	heat stress
	mutation
	ONSEN
	retrotransposon
発行日	2016-12
出版者	日本遺伝学会
出版者	The Genetics Society of Japan
収録物名	Genes & Genetic Systems
巻(号)	91(6)
ページ	293-299
抄録	Natural accessions are used for studying intraspecies genetic variation in the model plant Arabidopsis thaliana in order to address fundamental questions of evolution. Transposable elements are responsible for a wide range of mutations and play significant roles in shaping a genome over evolutionary time. In the present study, we aimed to characterize ONSEN, a heat-activated long terminal repeat (LTR) retrotransposon, in natural A. thaliana accessions. Southern blot analysis demonstrated that ONSEN was present in all the studied accessions, but the copy number was diverse. Olympia-1 contained a single ONSEN copy, located in the centromere of Chromosome 3. The stop codons in Olympia-1 ONSEN presumably abolish integrase activity, which in turn presumably renders the retrotransposon non-functional. Hybridization of Col-0 with Olympia-1 showed that several ONSEN copies in Col-0 were activated by heat stress and maintained their transpositional activity in the progeny.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1341-7568
EISSN	1880-5779
関連情報	PMID 27980240
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1266/ggs.16-00045