α-TCP複合化4-META/MMA-TBBレジン上へのrhBMP-2による骨増生

α-TCP

北海道歯学会

北海道歯学雑誌

It is the case that 4-META/MMA-TBB resin (Super-Bond, Sun Medical) has been used for root-end sealing following apico-ectomy and the bonding treatment of vertical root fractures because of the high adhesive property and biocompatibility. Furthermore, 4-META/MMA-TBB resin containing α-TCP was covered with augmentation of bone during the healing of a bone marrow defect and showed partially direct contact with new bone. In this study, we evaluated bone formation ratio and the direct contact ratio following BMP application on 4-META/MMA-TBB resin containing α-TCP on skulls of rats. The skin flap was elevated and periosteum was peeled out of the skull parietal bone of rats. One cup of polymer powder containing 0wt% or 60wt% α-TCP of Super-Bond was mixed with four drops of a monomer liquid and one drop of an initiator, and was applied to the bone. Two groups were prepared : one in which the resin was covered with a collagen sponge infused with rhBMP-2, and one in which it was not. Histological specimens were prepared at 4 and 8 weeks postoperation, and the sections were stained with H-E. New bone formation and histological measurements were histologically observed in bone coverage ratio over the resin and the direct contact ratio with new bone and the resin itself. As a result, bone coverage ratio and the direct contact ratio were significantly higher in the case of the resin containing α-TCP more than in case of the resin only. Bone coverage ratio in case of the resin containing α-TCP was 14.2(6.0-23.5)%. On the other hand, entire bone coverage was induced by application of BMP on the resin containing α-TCP or the resin only. The direct contact ratio in case of BMP application on the resin containing α-TCP was significantly higher than that in case of BMP application on resin only. In summary, it was suggested that the BMP application on the resin containing α-TCP, can induce bone growth covering the entire surface of the resin and increase the direct contact ratio between the resin and new bone.