A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation

Katayama, Shota; Moriguchi, Tetsuo; Ohtsu, Naoki; Kondo, Toru

A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation

Katayama, Shota; Moriguchi, Tetsuo; Ohtsu, Naoki et al.

Angewandte chemie. international edition, 2016, 55(22), 6452-6456

Permalink : https://hdl.handle.net/2115/65578

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/65578
タイトル	A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation
著者	Katayama, Shota ; Moriguchi, Tetsuo ; Ohtsu, Naoki ; Kondo, Toru
著者名 Katayama, Shota
e-Rad_Researcher 40323571 著者名 Moriguchi, Tetsuo
著者名 Ohtsu, Naoki
e-Rad_Researcher 30270573 著者名 Kondo, Toru
言語	英語
キーワード	CRISPR/Cas9
	epigenetics
	gene expression
	gene technology
	synthetic biology
	DNA methylation
	mRNA
発行日	2016-05-23
出版者	Wiley
収録物名	Angewandte chemie. international edition
巻(号)	55(22)
ページ	6452-6456
抄録	Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker βIII-tubulin.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	This is the peer reviewed version of the following article: S. Katayama, T. Moriguchi, N. Ohtsu, T. Kondo, Angew. Chem. Int. Ed. 2016, 55, 6452, which has been published in final form at http://dx.doi.org/10.1002/anie.201601708. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN	1433-7851
NCID	AA0052535X
関連情報	PMID 27079176
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/anie.201601708