Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study

Kawakubo, Kazumichi; Ohnishi, Shunsuke; Hatanaka, Yutaka; Hatanaka, Kanako C.; Hosono, Hidetaka; Kubota, Yoshimasa; Kamiya, Mako; Kuwatani, Masaki; Kawakami, Hiroshi; Urano, Yasuteru; Sakamoto, Naoya

Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study

Kawakubo, Kazumichi; Ohnishi, Shunsuke; Hatanaka, Yutaka et al.

Molecular imaging and biology, 2016, 18(3), 463-471

Permalink : https://hdl.handle.net/2115/65837

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アクセス権	open access
URI	https://hdl.handle.net/2115/65837
タイトル	Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study
その他のタイトル	Fluorescence evaluation of EUS-FNA specimens
著者	Kawakubo, Kazumichi ; Ohnishi, Shunsuke ; Hatanaka, Yutaka ; Hatanaka, Kanako C. ; Hosono, Hidetaka ; Kubota, Yoshimasa ; Kamiya, Mako ; Kuwatani, Masaki ; Kawakami, Hiroshi ; Urano, Yasuteru ; Sakamoto, Naoya
著者名 Kawakubo, Kazumichi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
e-Rad_Researcher 30589924 著者名 Hatanaka, Yutaka
著者名 Hatanaka, Kanako C.
著者名 Hosono, Hidetaka
著者名 Kubota, Yoshimasa
e-Rad_Researcher 90596462 著者名 Kamiya, Mako
e-Rad_Researcher 50431375 著者名 Kuwatani, Masaki
e-Rad_Researcher 80399823 著者名 Kawakami, Hiroshi
著者名 Urano, Yasuteru
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Endoscopic ultrasound-guided fine needle aspiration
	Pancreatic tumor
	Fluorescent imaging
	Enzymatically activatable fluorescence probe
	Rapid on-site evaluation
発行日	2016-06
出版者	Springer
収録物名	Molecular imaging and biology
巻(号)	18(3)
ページ	463-471
抄録	Purpose: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). Procedures: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. Results: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). Conclusions: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-015-0898-5
PISSN	1536-1632
関連情報	PMID 26431952
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11307-015-0898-5