Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells

Ukita, Mayumi; Yamaguchi, Taihiko; Ohata, Noboru; Tamura, Masato

Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells

Ukita, Mayumi; Yamaguchi, Taihiko; Ohata, Noboru et al.

Journal of cellular biochemistry, 2016, 117(6), 1419-1428

Permalink : https://hdl.handle.net/2115/65842

このアイテムのアクセス数:1,150件（2026-08-10 01:28 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Ukita_HUSCAP	pdf	1.02 MB	1,258

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/65842
タイトル	Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells
著者	Ukita, Mayumi ; Yamaguchi, Taihiko ; Ohata, Noboru ; Tamura, Masato
著者名 Ukita, Mayumi
e-Rad_Researcher 90200617 著者名 Yamaguchi, Taihiko
e-Rad_Researcher 60002185 著者名 Ohata, Noboru
e-Rad_Researcher 30236757 著者名 Tamura, Masato
言語	英語
キーワード	sclerostin
	adipocyte
	osteocyte
	Sost
発行日	2016-06
出版者	Wiley-Blackwell
収録物名	Journal of cellular biochemistry
巻(号)	117(6)
ページ	1419-1428
抄録	Sclerostin, a secreted protein encoded by the Sost gene, is produced by osteocytes and is inhibited by osteoblast differentiation and bone formation. Recently, a functional association between bone and fat tissue has been suggested, and a correlation between circulating sclerostin levels and lipid metabolism has been reported in humans. However, the effects of sclerostin on adipogenesis remain unexplored. In the present study, we examined the role of sclerostin in regulating adipocyte differentiation using 3T3-L1 preadipocytes. In these cells, sclerostin enhanced adipocyte-specific gene expression and the accumulation of lipid deposits. Sclerostin also upregulated CCAAT/enhancer binding protein expression but not cell proliferation and caspase-3/7 activities. Sclerostin also attenuated canonical Wnt3a-inhibited adipocyte differentiation. Recently, the transcriptional modulator TAZ has been involved in the canonical Wnt signaling pathway. Sclerostin reduced TAZ-responsive transcriptional activity and TAZ-responsive gene expression. Transfection of 3T3-L1 cells with TAZ siRNA increased the lipid deposits and adipogenic gene expression. These results show that sclerostin upregulates adipocyte differentiation in 3T3-L1 cells, suggesting a possible role for the osteocyte-derived sclerostin as a regulator of fat metabolism and as a reciprocal regulator of bone and adipose tissues metabolism. J. Cell. Biochem. 117: 1419-1428, 2016. (c) 2015 Wiley Periodicals, Inc.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: [Sclerostin enhances adipocyte differentiation in 3T3-L1 cells], which has been published in final form at [http://dx.doi.org/10.1002/jcb.25432]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN	0730-2312
EISSN	1097-4644
関連情報	PMID 26553151
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/jcb.25432