Role of ATF5 in the invasive potential of diverse human cancer cell lines

Nukuda, Akihiro; Endoh, Hiroki; Yasuda, Motoaki; Mizutani, Takeomi; Kawabata, Kazushige; Haga, Hisashi

Role of ATF5 in the invasive potential of diverse human cancer cell lines

Nukuda, Akihiro; Endoh, Hiroki; Yasuda, Motoaki et al.

Biochemical and biophysical research communications, 2016, 474(3), 509-514

Permalink : https://hdl.handle.net/2115/65863

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アクセス権	open access
URI	https://hdl.handle.net/2115/65863
タイトル	Role of ATF5 in the invasive potential of diverse human cancer cell lines
著者	Nukuda, Akihiro ; Endoh, Hiroki ; Yasuda, Motoaki ; Mizutani, Takeomi ; Kawabata, Kazushige ; Haga, Hisashi
著者名 Nukuda, Akihiro
著者名 Endoh, Hiroki
著者名 Yasuda, Motoaki
著者名 Mizutani, Takeomi
著者名 Kawabata, Kazushige
e-Rad_Researcher 00292045 著者名 Haga, Hisashi
言語	英語
キーワード	Activating transcription factor 5
	Integrin-alpha 2
	Integrin-beta 1
	Invasion
	Spindle morphology
発行日	2016-06-03
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	474(3)
ページ	509-514
抄録	Activating transcription factor 5 (ATF5) is a member of the ATF/CAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-alpha 2 and integrin-beta 1 expression and that the depletion of integrin-alpha 2 or integrin-beta 1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-alpha 2 and integrin-beta 1 in several human cancer cell lines. (C) 2016 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0006-291X
関連情報	PMID 27125458
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2016.04.131