Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis

Miyamoto, Shuichi; Ohnishi, Shunsuke; Onishi, Reizo; Tsuchiya, Ikuki; Hosono, Hidetaka; Katsurada, Takehiko; Yamahara, Kenichi; Takeda, Hiroshi; Sakamoto, Naoya

Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis

Miyamoto, Shuichi; Ohnishi, Shunsuke; Onishi, Reizo et al.

American journal of translational research, 2017, 9(3), 940-952

Permalink : https://hdl.handle.net/2115/66142

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アクセス権	open access
URI	https://hdl.handle.net/2115/66142
タイトル	Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis
著者	Miyamoto, Shuichi ; Ohnishi, Shunsuke ; Onishi, Reizo ; Tsuchiya, Ikuki ; Hosono, Hidetaka ; Katsurada, Takehiko ; Yamahara, Kenichi ; Takeda, Hiroshi ; Sakamoto, Naoya
著者名 Miyamoto, Shuichi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Onishi, Reizo
著者名 Tsuchiya, Ikuki
著者名 Hosono, Hidetaka
著者名 Katsurada, Takehiko
著者名 Yamahara, Kenichi
e-Rad_Researcher 60261294 著者名 Takeda, Hiroshi
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Mesenchymal stem cells
	amnion
	conditioned medium
	trinitrobenzene sulfonic acid
	colitis
発行日	2017
出版者	e-Century Publishing Corporation
収録物名	American journal of translational research
巻(号)	9(3)
ページ	940-952
抄録	Cell therapy with mesenchymal stem cells (MSCs) is expected to provide a new strategy for the treatment of inflammatory bowel disease (IBD). Large amounts of MSCs can be obtained from human amnion. Therefore, we investigated the effect of transplantation of human amnion-derived MSCs (hAMSCs) or enema of conditioned medium (CM) from hAMSCs into rats with 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. In the first experiment, 10-week-old male Sprague-Dawley rats were intravenously injected with hAMSCs (1 × 10^6 cells) 3 h after rectal administration of TNBS (45 mg/kg). In the second experiment, rats with TNBS-induced colitis received CM by enema into the colon for 3 days. Colitis was investigated by endoscopy, histology, immunohistochemistry, and by measuring mRNA expression of inflammatory mediators. Administration of hAMSCs or CM enema significantly improved the endoscopic score. In addition, these two interventions resulted in significantly decreased infiltration of neutrophils and monocytes/macrophages and decreased expression levels of TNF-α, CXCL1, and CCL2. In conclusion, transplantation of hAMSCs and CM enema provided significant improvement in rats with TNBS-induced colitis. CM from hAMSCs and hAMSCs may be new strategies for the treatment of IBD.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 4.0 International
PISSN	1943-8141