17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon

Ijiri, Shigeho; Shibata, Yasushi; Takezawa, Nonoha; Kazeto, Yukinori; Takatsuka, Naoki; Kato, Erika; Hagihara, Seishi; Ozaki, Yuichi; Adachi, Shinji; Yamauchi, Kohei; Nagahama, Yoshitaka

17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon

Ijiri, Shigeho; Shibata, Yasushi; Takezawa, Nonoha et al.

Endocrinology, 2017, 158(3), 627-639

Permalink : https://hdl.handle.net/2115/66278

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アクセス権	open access
URI	https://hdl.handle.net/2115/66278
タイトル	17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon
著者	Ijiri, Shigeho ; Shibata, Yasushi ; Takezawa, Nonoha ; Kazeto, Yukinori ; Takatsuka, Naoki ; Kato, Erika ; Hagihara, Seishi ; Ozaki, Yuichi ; Adachi, Shinji ; Yamauchi, Kohei ; Nagahama, Yoshitaka
e-Rad_Researcher 90425421 著者名 Ijiri, Shigeho
著者名 Shibata, Yasushi
著者名 Takezawa, Nonoha
著者名 Kazeto, Yukinori
著者名 Takatsuka, Naoki
著者名 Kato, Erika
著者名 Hagihara, Seishi
著者名 Ozaki, Yuichi
e-Rad_Researcher 40231930 著者名 Adachi, Shinji
著者名 Yamauchi, Kohei
著者名 Nagahama, Yoshitaka
言語	英語
キーワード	17α,20β-dihydroxy-4-pregnen-3-one
	20β-hydroxysteroid dehydrogenase
	17β-hydroxysteroid dehydrogenase
	Oocyte maturation
	Granulosa, Masu salmon
	Oncorhynchus masou
発行日	2017-03
出版者	Endocrine Society
収録物名	Endocrinology
巻(号)	158(3)
ページ	627-639
抄録	The maturation-inducing hormone 17(alpha), 20(beta)-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. Although carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase (CR/20b-HSD) was reported to convert 17 alpha-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20 beta-HSD messenger RNA (mRNA) was not upregulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the current study aimed to identify the specific 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA sequencing was performed on granulosa layers that were isolated from ovarian follicles at 1 month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and similar to 12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified 1 contig (#f103496)as similar to 17 beta-hydroxysteroid dehydrogenase type 12 (hsd17 beta 12); however, because the full-length #f103496 sequence was different from hsd17 beta 12, it was termed hsd17 beta 12-like (hsd17 beta 12l). We found that mammalian cells transfected with full-length hsd171 beta 2l exhibited considerable 20b-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17 beta 12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocytematuration. The levels of hsd17 beta 12lmRNA were also considerably increased in granulosa layers in which 20 beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17 beta 12l, not CR/20 beta-HSD, is the 20 beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocytematuration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0013-7227
EISSN	1945-7170
NCID	AA00635074
関連情報	PMID 27967235
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1210/en.2016-1349