Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

Kobayashi, Takahiko; Ishida, Junich; Shimizu, Yuichi; Kawakami, Hiroshi; Suda, Goki; Muranaka, Tetsuhito; Komatsu, Yoshito; Asaka, Masahiro; Sakamoto, Naoya

Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

Kobayashi, Takahiko; Ishida, Junich; Shimizu, Yuichi et al.

Tumor biology, 2017, 39(3)

Permalink : https://hdl.handle.net/2115/66569

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/66569
タイトル	Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer
著者	Kobayashi, Takahiko ; Ishida, Junich ; Shimizu, Yuichi ; Kawakami, Hiroshi ; Suda, Goki ; Muranaka, Tetsuhito ; Komatsu, Yoshito ; Asaka, Masahiro ; Sakamoto, Naoya
e-Rad_Researcher 80333607 著者名 Kobayashi, Takahiko
著者名 Ishida, Junich
e-Rad_Researcher 90333608 著者名 Shimizu, Yuichi
e-Rad_Researcher 80399823 著者名 Kawakami, Hiroshi
e-Rad_Researcher 20447460 著者名 Suda, Goki
著者名 Muranaka, Tetsuhito
e-Rad_Researcher 60333598 著者名 Komatsu, Yoshito
e-Rad_Researcher 10113507 著者名 Asaka, Masahiro
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Gastric cancer
	RNA-binding motif 5
	tumor suppressor gene
	tumor progression
発行日	2017-03-01
出版者	SAGE Publications
収録物名	Tumor biology
巻(号)	39(3)
抄録	RNA-binding motif 5 is a putative tumor suppressor gene that modulates cell cycle arrest and apoptosis. We recently demonstrated that RNA-binding motif 5 inhibits cell growth through the p53 pathway. This study evaluated the clinical significance of RNA-binding motif 5 expression in gastric cancer and the effects of altered RNA-binding motif 5 expression on cancer biology in gastric cancer cells. RNA-binding motif 5 protein expression was evaluated by immunohistochemistry using the surgical specimens of 106 patients with gastric cancer. We analyzed the relationships of RNA-binding motif 5 expression with clinicopathological parameters and patient prognosis. We further explored the effects of RNA-binding motif 5 downregulation with short hairpin RNA on cell growth and p53 signaling in MKN45 gastric cancer cells. Immunohistochemistry revealed that RNA-binding motif 5 expression was decreased in 29 of 106 (27.4%) gastric cancer specimens. Decreased RNA-binding motif 5 expression was correlated with histological differentiation, depth of tumor infiltration, nodal metastasis, tumor-node-metastasis stage, and prognosis. RNA-binding motif 5 silencing enhanced gastric cancer cell proliferation and decreased p53 transcriptional activity in reporter gene assays. Conversely, restoration of RNA-binding motif 5 expression suppressed cell growth and recovered p53 transactivation in RNA-binding motif 5-silenced cells. Furthermore, RNA-binding motif 5 silencing reduced the messenger RNA and protein expression of the p53 target gene p21. Our results suggest that RNA-binding motif 5 downregulation is involved in gastric cancer progression and that RNA-binding motif 5 behaves as a tumor suppressor gene in gastric cancer.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 3.0 Unported
PISSN	1010-4283
関連情報	PMID 28347247
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1177/1010428317694547