open access

https://hdl.handle.net/2115/66902

Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens)

英語

Shrew

2016-07-30

Mary Ann Liebert, Inc.

Vector-Borne and Zoonotic Diseases

16(7)

468-475

Although based on very limited M and L segment sequences, Artybash virus (ARTV) was proposed previously as a unique hantavirus harbored by the Laxmann’s shrew (Sorex caecutiens). To verify this conjecture, lung tissues from 68 Laxmann’s shrews, captured during 2006 to 2014 in eastern Siberia, Russia, and Hokkaido, Japan, were analyzed for ARTV RNA using RT-PCR. ARTV RNA was detected in six Laxmann’s shrews. Pairwise alignment and comparison of partial- and full-length S, M, and L segment sequences from these Laxmann’s shrews, as well as phylogenetic analyses, using maximum likelihood and Bayesian methods indicated that ARTV was distinct from other soricine shrew-borne hantaviruses and representative hantaviruses harbored by rodents, moles, and bats. Taxonomic identity of the ARTV-infected Laxmann’s shrews was confirmed by full-length cytochrome b mitochondrial DNA sequence analysis. Our data indicate that the hantavirus previously known as Amga virus (MGAV) represents genetic variants of ARTV. Thus, the previously proposed designation of ARTV/MGAV should be replaced by ARTV

学術雑誌論文

Accepted Manuscript

Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/vbz.2015.1903

1530-3667

1557-7759