Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts.

Yamamori, Tohru; Sasagawa, Tomoya; Ichii, Osamu; Hiyoshi, Mie; Bo, Tomoki; Yasui, Hironobu; Kon, Yasuhiro; Inanami, Osamu

Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts.

Yamamori, Tohru; Sasagawa, Tomoya; Ichii, Osamu et al.

Journal of radiation research, 2016, 58(3), 292-301

Permalink : https://hdl.handle.net/2115/66942

このアイテムのアクセス数:956件（2026-08-16 04:00 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
inal	pdf	781 KB	534

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/66942
タイトル	Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts.
著者	Yamamori, Tohru ; Sasagawa, Tomoya ; Ichii, Osamu ; Hiyoshi, Mie ; Bo, Tomoki ; Yasui, Hironobu ; Kon, Yasuhiro ; Inanami, Osamu
e-Rad_Researcher 00512675 著者名 Yamamori, Tohru
著者名 Sasagawa, Tomoya
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
著者名 Hiyoshi, Mie
著者名 Bo, Tomoki
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
言語	英語
キーワード	ionizing radiation
	cellular radioresponse
	mitochondria
	mitochondrial biogenesis
	cell cycle arrest
	cellular senescence
発行日	2016-12-14
出版者	Oxford University Press
収録物名	Journal of radiation research
巻(号)	58(3)
ページ	292-301
抄録	Mitochondria strongly contribute to the maintenance of cellular integrity through various mechanisms, including oxidative adenosine triphosphate production and calcium homeostasis regulation. Therefore, proper regulation of the abundance, distribution and activity of mitochondria is crucial for the maintenance of cellular homeostasis. Previous studies have shown that ionizing radiation (IR) alters mitochondrial functions, suggesting that mitochondria are likely to be an important target of IR. Though IR reportedly influences cellular mitochondrial abundance, the mechanism remains largely unknown. In this study, we examined how IR influences mitochondrial abundance in mouse fibroblasts. When mouse NIH/3T3 cells were exposed to X-rays, a time-dependent increase was observed in mitochondrial DNA (mtDNA) and mitochondrial mass, indicating radiation-induced upregulation of mitochondrial abundance. Meanwhile, not only did we not observe a significant change in autophagic activity after irradiation, but in addition, IR hardly influenced the expression of two mitochondrial proteins, cytochrome c oxidase subunit IV and cytochrome c, or the mRNA expression of Polg, a component of DNA polymerase γ. We also observed that the expression of transcription factors involved in mitochondrial biogenesis was only marginally affected by IR. These data imply that radiation-induced upregulation of mitochondrial abundance is an event independent of macroautophagy and mitochondrial biogenesis. Furthermore, we found evidence that IR induced long-term cell cycle arrest and cellular senescence, indicating that these events are involved in regulating mitochondrial abundance. Considering the growing significance of mitochondria in cellular radioresponses, we believe the present study provides novel insights into understanding the effects of IR on mitochondria.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 4.0 International
PISSN	1349-9157
関連情報	PMID 27974504
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1093/jrr/rrw113