Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing

Kikuchi, Yusuke; Hijikata, Nowaki; Ohtomo, Ryo; Handa, Yoshihiro; Kawaguchi, Masayoshi; Saito, Katsuharu; Masuta, Chikara; Ezawa, Tatsuhiro

Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing

Kikuchi, Yusuke; Hijikata, Nowaki; Ohtomo, Ryo et al.

New Phytologist, 2016, 211(4), 1202-1208

Permalink : https://hdl.handle.net/2115/67113

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アクセス権	open access
URI	https://hdl.handle.net/2115/67113
タイトル	Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing
著者	Kikuchi, Yusuke ; Hijikata, Nowaki ; Ohtomo, Ryo ; Handa, Yoshihiro ; Kawaguchi, Masayoshi ; Saito, Katsuharu ; Masuta, Chikara ; Ezawa, Tatsuhiro
著者名 Kikuchi, Yusuke
著者名 Hijikata, Nowaki
著者名 Ohtomo, Ryo
著者名 Handa, Yoshihiro
著者名 Kawaguchi, Masayoshi
著者名 Saito, Katsuharu
著者名 Masuta, Chikara
e-Rad_Researcher 40273213 著者名 Ezawa, Tatsuhiro
言語	英語
キーワード	arbuscular mycorrhiza
	aquaglyceroporin
	aquaporin
	cucumber mosaic virus
	polyphosphate translocation
	Rhizophagus clarus, transpiration
	virus-induced gene silencing
発行日	2016-09
出版者	Wiley-Blackwell
収録物名	New Phytologist
巻(号)	211(4)
ページ	1202-1208
抄録	Arbuscular mycorrhizal fungi translocate polyphosphate through hyphae over a long distance to deliver to the host. More than three decades ago, suppression of host transpiration was found to decelerate phosphate delivery of the fungal symbiont, leading us to hypothesize that transpiration provides a primary driving force for polyphosphate translocation, probably via creating hyphal water flow in which fungal aquaporin(s) may be involved. The impact of transpiration suppression on polyphosphate translocation through hyphae of Rhizophagus clarus was evaluated. An aquaporin gene expressed in intraradical mycelia was characterized and knocked down by virus-induced gene silencing to investigate the involvement of the gene in polyphosphate translocation. Rhizophagus clarus aquaporin 3 (RcAQP3) that was most highly expressed in intraradical mycelia encodes an aquaglyceroporin responsible for water transport across the plasma membrane. Knockdown of RcAQP3 as well as the suppression of host transpiration decelerated polyphosphate translocation in proportion to the levels of knockdown and suppression, respectively. These results provide the first insight into the mechanism underlying long-distance polyphosphate translocation in mycorrhizal associations at the molecular level, in which host transpiration and the fungal aquaporin play key roles. A hypothetical model of the translocation is proposed for further elucidation of the mechanism.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: [Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis : application of virus-induced gene silencing], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN	0028-646X
EISSN	1469-8137
関連情報	PMID 27136716
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/nph.14016