Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)

Kakinuma, Makoto; Suzuki, Kohei; Iwata, Shintaro; Coury, Daniel A.; Iwade, Shouei; Mikami, Koji

Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)

Kakinuma, Makoto; Suzuki, Kohei; Iwata, Shintaro et al.

Fisheries Science, 2016, 82(1), 171-184

Permalink : https://hdl.handle.net/2115/67198

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アクセス権	open access
URI	https://hdl.handle.net/2115/67198
タイトル	Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
著者	Kakinuma, Makoto ; Suzuki, Kohei ; Iwata, Shintaro ; Coury, Daniel A. ; Iwade, Shouei ; Mikami, Koji
著者名 Kakinuma, Makoto
著者名 Suzuki, Kohei
著者名 Iwata, Shintaro
著者名 Coury, Daniel A.
著者名 Iwade, Shouei
e-Rad_Researcher 40222319 著者名 Mikami, Koji
言語	英語
キーワード	cDNA cloning
	DUR3
	Gene expression
	Pyropia yezoensis
	Rhodophyta
	Sodium solute symporter
	Urea transporter
発行日	2016-01
出版者	日本水産学会
収録物名	Fisheries Science
巻(号)	82(1)
ページ	171-184
抄録	DUR3 proteins mediate high-affinity transport of exogenous and endogenous urea. Although two DUR3-like genes (PyDUR3.1/3.2) have been identified in Pyropia yezoensis, a BLAST search using these sequences against the P. yezoensis EST database suggested the existence of another DUR3-like gene (PyDUR3.3). In this study, the PyDUR3.3 gene was isolated and characterized and compared to PyDUR3.1/3.2 genes. The predicted length of PyDUR3.3 was 679 amino acids, which included 15 transmembrane domains. An amino acid sequence alignment of algal, plant, and yeast DUR3 proteins showed that PyDUR3.3 was more similar to PyDUR3.2 than to other DUR3 proteins, including PyDUR3.1. Exon–intron structures of PyDUR3.3 and PyDUR3.2 were also closely related to each other, which clearly differed from that of PyDUR3.1. Expression analysis showed that PyDUR3.3 mRNA levels were extremely high in sporophytes, regardless of the nutrient condition, compared to gametophytes. On the other hand, expression of PyDUR3.2 and PyDUR3.1 was high in the gametophytes and sporophytes, respectively, and was induced by nutrient starvation. These results suggest that expression of PyDUR3.3/3.2/3.1 depends on the life history phase as well as the nutrient conditions, and that PyDUR3.3 and PyDUR3.2 are paralogues specifically differentiated in function and life history phase.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-015-0947-7
PISSN	0919-9268
EISSN	1444-2906
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s12562-015-0947-7