The phenotype of infiltrating macrophages influences arteriosclerotic plaque vulnerability in the carotid artery

Cho, Kyu Yong; Miyoshi, Hideaki; Kuroda, Satoshi; Yasuda, Hiroshi; Kamiyama, Kenji; Nakagawara, Joji; Takigami, Masayoshi; Kondo, Takuma; Atsumi, Tatsuya

The phenotype of infiltrating macrophages influences arteriosclerotic plaque vulnerability in the carotid artery

Cho, Kyu Yong; Miyoshi, Hideaki; Kuroda, Satoshi et al.

Journal of stroke and cerebrovascular diseases, 2013, 22(7), 910-918

Permalink : https://hdl.handle.net/2115/67265

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アクセス権	open access
URI	https://hdl.handle.net/2115/67265
タイトル	The phenotype of infiltrating macrophages influences arteriosclerotic plaque vulnerability in the carotid artery
著者	Cho, Kyu Yong ; Miyoshi, Hideaki ; Kuroda, Satoshi ; Yasuda, Hiroshi ; Kamiyama, Kenji ; Nakagawara, Joji ; Takigami, Masayoshi ; Kondo, Takuma ; Atsumi, Tatsuya
著者名 Cho, Kyu Yong
e-Rad_Researcher 30360902 著者名 Miyoshi, Hideaki
e-Rad_Researcher 10301904 著者名 Kuroda, Satoshi
著者名 Yasuda, Hiroshi
著者名 Kamiyama, Kenji
著者名 Nakagawara, Joji
著者名 Takigami, Masayoshi
著者名 Kondo, Takuma
e-Rad_Researcher 20301905 著者名 Atsumi, Tatsuya
言語	英語
キーワード	Atherosclerosis
	carotid artery disease
	inflammation
	macrophage
	stroke
発行日	2013-10
出版者	Elsevier
収録物名	Journal of stroke and cerebrovascular diseases
巻(号)	22(7)
ページ	910-918
抄録	Background: Proinflammatory (M1) macrophages and anti-inflammatory (M2) macrophages have been identified in atherosclerotic plaques. While these macrophages have been speculated to be related to plaque vulnerability, there are limited studies investigating this relationship. Therefore, we examined the association between macrophage phenotype (M1 versus M2) and plaque vulnerability and clinical events. Methods: Patients undergoing carotid endarterectomy received an ultrasound of the carotid artery before surgery. Plaques were processed for analysis by immunohistochemistry, Western blotting, and real-time polymerase chain reaction studies. Medical history and clinical data were obtained from medical records. Results: Patients were divided into 2 groups: those suffering from acute ischemic attack (symptomatic, n = 31) and those that did not present with symptoms (asymptomatic, n = 34). Ultrasound analysis revealed that plaque vulnerability was greater in the symptomatic group (P= .033; Chi-square test). Immunohistochemistry revealed that plaques from the symptomatic group had a greater concentration of M1 macrophages (CD68-, CD11c-positive) while plaques from the asymptomatic group had more M2 macrophages (CD163-positive). This observation was confirmed by Western blotting. Characterization by real-time polymerase chain reaction studies revealed that plaques from the symptomatic group had increased expression of the M1 markers CD68 and CD11c, as well as monocyte chemoattractive protein-1, interleukin-6, and matrix metalloproteinase-9. In addition, more M1 macrophages expressed in unstable plaques were defined by ultrasound analysis, while more M2 macrophages were expressed in stable plaques. Conclusions: Our data show that M1 macrophage content of atherosclerotic plaques is associated with clinical incidence of ischemic stroke and increased inflammation or fibrinolysis. We also show the benefits of using ultrasound to evaluate vulnerability in the plaques.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	1052-3057
関連情報	PMID 23273713
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.jstrokecerebrovasdis.2012.11.020