Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses

Yoshida, Reiko; Muramatsu, Shino; Akita, Hiroshi et al.

Journal of Infectious Diseases, 2016, 214(Suppl. 3), S185-S191

Permalink : https://hdl.handle.net/2115/67314

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/67314
タイトル
Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses
著者
Yoshida, Reiko ; Muramatsu, Shino ; Akita, Hiroshi ; Saito, Yuji ; Kuwahara, Miwa ; Kato, Daisuke ; Changula, Katendi ; Miyamoto, Hiroko ; Kajihara, Masahiro ; Manzoor, Rashid ; Furuyama, Wakako ; Marzi, Andrea ; Feldmann, Heinz ; Mweene, Aaron ; Masumu, Justin ; Kapeteshi, Jimmy ; Muyembe-Tamfum, Jean-Jacques ; Takada, Ayato
著者名
Yoshida, Reiko
著者名
Muramatsu, Shino
著者名
Akita, Hiroshi
著者名
Saito, Yuji
著者名
Kuwahara, Miwa
著者名
Kato, Daisuke
著者名
Changula, Katendi
著者名
Miyamoto, Hiroko
著者名
Kajihara, Masahiro
著者名
Manzoor, Rashid
著者名
Furuyama, Wakako
著者名
Marzi, Andrea
著者名
Feldmann, Heinz
著者名
Mweene, Aaron
著者名
Masumu, Justin
著者名
Kapeteshi, Jimmy
著者名
Muyembe-Tamfum, Jean-Jacques
e-Rad_Researcher
10292062
著者名
Takada, Ayato
言語
英語
キーワード
Ebola virus
ebolavirus
EVD
filovirus
nucleoprotein
monoclonal antibody
immunochromatography assay
diagnosis
発行日
2016-10-15
出版者
Oxford University Press
収録物名
Journal of Infectious Diseases
巻(号)
214(Suppl. 3)
ページ
S185-S191
抄録
The latest outbreak of Ebola virus disease (EVD) in West Africa has highlighted the urgent need for the development of rapid and reliable diagnostic assays. We used monoclonal antibodies specific to the ebolavirus nucleoprotein to develop an immunochromatography (IC) assay (QuickNavi-Ebola) for rapid diagnosis of EVD. The IC assay was first evaluated with tissue culture supernatants of infected Vero E6 cells and found to be capable of detecting 10(3)-10(4) focus-forming units/mL of ebolaviruses. Using serum samples from experimentally infected nonhuman primates, we confirmed that the assay could detect the viral antigen shortly after disease onset. It was also noted that multiple species of ebolaviruses could be detected by the IC assay. Owing to the simplicity of the assay procedure and absence of requirements for special equipment and training, QuickNavi-Ebola is expected to be a useful tool for rapid diagnosis of EVD.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record 214(Suppl.3): S185-S191, 2016 is available online at: https://doi.org/10.1093/infdis/jiw252.
PISSN
0022-1899
NCID
AA0070010X
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1093/infdis/jiw252