Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

Kihara, Akihiko; Saburi, Wataru; Wakuta, Shinji; Kim, Myung-Hee; Hamada, Shigeki; Ito, Hiroyuki; Imai, Ryozo; Matsui, Hirokazu

Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

Kihara, Akihiko; Saburi, Wataru; Wakuta, Shinji et al.

Bioscience, Biotechnology, and Biochemistry, 2011, 75(9), 1740-1745

Permalink : https://hdl.handle.net/2115/67570

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アクセス権	open access
URI	https://hdl.handle.net/2115/67570
タイトル	Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)
著者	Kihara, Akihiko ; Saburi, Wataru ; Wakuta, Shinji ; Kim, Myung-Hee ; Hamada, Shigeki ; Ito, Hiroyuki ; Imai, Ryozo ; Matsui, Hirokazu
著者名 Kihara, Akihiko
e-Rad_Researcher 00598089 著者名 Saburi, Wataru
著者名 Wakuta, Shinji
著者名 Kim, Myung-Hee
著者名 Hamada, Shigeki
著者名 Ito, Hiroyuki
著者名 Imai, Ryozo
e-Rad_Researcher 90109504 著者名 Matsui, Hirokazu
言語	英語
キーワード	nucleoside diphosphate kinase
	subcellular localization
	ping-pong mechanism
	gene expression
	enzyme characteristics
発行日	2011-09
出版者	Taylor & Francis
収録物名	Bioscience, Biotechnology, and Biochemistry
巻(号)	75(9)
ページ	1740-1745
抄録	Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme that catalyzes the transfer of the γ-phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. In this study, we examined the subcellular localization, tissue-specific gene expression, and enzymatic characteristics of three rice NDPK isozymes (OsNDPK1-OsNDPK3). Sequence comparison of the three OsNDPKs suggested differential subcellular localization. Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol. Expression analysis indicated that all the OsNDPKs are expressed in the leaf, leaf sheath, and immature seeds, except for OsNDPK1, in the leaf sheath. Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1. In ATP formation, all the OsNDPKs displayed lower K(m) values for the second substrate, ADP, than for the first substrate, NTP, and showed lowest and highest K(m) values for GTP and CTP respectively.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2011 Sep, available online: http://wwww.tandfonline.com/10.1271/bbb.110285.
PISSN	0916-8451
EISSN	1347-6947
NCID	AA10824164
関連情報	PMID 21897044
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1271/bbb.110285