Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus

Phongphaew, Wallaya; Kobayashi, Shintaro; Sasaki, Michihito; Carr, Michael; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi

Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus

Phongphaew, Wallaya; Kobayashi, Shintaro; Sasaki, Michihito et al.

Virus Research, 2017, 228, 114-123

Permalink : https://hdl.handle.net/2115/67761

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アクセス権	open access
URI	https://hdl.handle.net/2115/67761
タイトル	Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus
著者	Phongphaew, Wallaya ; Kobayashi, Shintaro ; Sasaki, Michihito ; Carr, Michael ; Hall, William W. ; Orba, Yasuko ; Sawa, Hirofumi
著者名 Phongphaew, Wallaya
e-Rad_Researcher 00634205 著者名 Kobayashi, Shintaro
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
e-Rad_Researcher 70769588 著者名 Carr, Michael
著者名 Hall, William W.
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
言語	英語
キーワード	VCP
	WNV replication
	Early steps
	Genome replication
発行日	2017-01-15
出版者	Elsevier
収録物名	Virus Research
巻(号)	228
ページ	114-123
抄録	Valosin-containing protein (VCP) is classified as a member of the type II AAA(+) ATPase protein family. VCP functions in several cellular processes, including protein degradation, membrane fusion, vesicular trafficking and disassembly of stress granules. Moreover, VCP is considered to play a role in the replication of several viruses, albeit through different mechanisms. In the present study, we have investigated the role of VCP in West Nile virus (WNV) infection. Endogenous VCP expression was inhibited using either VCP inhibitors or by siRNA knockdown. It could be shown that the inhibition of endogenous VCP expression significantly inhibited WNV infection. The entry assay revealed that silencing of endogenous VCP caused a significant reduction in the expression levels of WNV-RNA compared to control siRNA-treated cells. This indicates that VCP may play a role in early steps either the binding or entry steps of the WNV life cycle. Using WNV virus like particles and WNV-DNA-based replicon, it could be demonstrated that perturbation of VCP expression decreased levels of newly synthesized WNV genomic RNA. These findings suggest that VCP is involved in early steps and during genome replication of the WNV life cycle.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0168-1702
関連情報	PMID 27914931
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.virusres.2016.11.029