Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor

Mikuni, Shintaro; Yamamoto, Johtaro; Horio, Takashi; Kinjo, Masataka

Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor

Mikuni, Shintaro; Yamamoto, Johtaro; Horio, Takashi et al.

International Journal of Molecular Sciences, 2017, 18(9), 1855

Permalink : https://hdl.handle.net/2115/67846

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/67846
タイトル	Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
著者	Mikuni, Shintaro ; Yamamoto, Johtaro ; Horio, Takashi ; Kinjo, Masataka
著者名 Mikuni, Shintaro
著者名 Yamamoto, Johtaro
著者名 Horio, Takashi
e-Rad_Researcher 70177971 著者名 Kinjo, Masataka
言語	英語
キーワード	raster image correlation spectroscopy
	green fluorescence protein
	nuclear receptor
	diffusion analysis
	transcription
発行日	2017-09
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	18(9)
ページ	1855
抄録	The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1422-0067
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms18091855