Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis

Goto, Shinya; Konnai, Satoru; Okagawa, Tomohiro; Nishimori, Asami; Maekawa, Naoya; Gondaira, Satoshi; Higuchi, Hidetoshi; Koiwa, Masateru; Tajima, Motoshi; Kohara, Junko; Ogasawara, Satoshi; Kato, Yukinari; Suzuki, Yasuhiko; Murata, Shiro; Ohashi, Kazuhiko

Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis

Goto, Shinya; Konnai, Satoru; Okagawa, Tomohiro et al.

Immunity, inflammation and disease, 2017, 5(3), 355-363

Permalink : https://hdl.handle.net/2115/67897

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アクセス権	open access
URI	https://hdl.handle.net/2115/67897
タイトル	Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis
著者	Goto, Shinya ; Konnai, Satoru ; Okagawa, Tomohiro ; Nishimori, Asami ; Maekawa, Naoya ; Gondaira, Satoshi ; Higuchi, Hidetoshi ; Koiwa, Masateru ; Tajima, Motoshi ; Kohara, Junko ; Ogasawara, Satoshi ; Kato, Yukinari ; Suzuki, Yasuhiko ; Murata, Shiro ; Ohashi, Kazuhiko
著者名 Goto, Shinya
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
著者名 Okagawa, Tomohiro
著者名 Nishimori, Asami
著者名 Maekawa, Naoya
著者名 Gondaira, Satoshi
著者名 Higuchi, Hidetoshi
e-Rad_Researcher 90094820 著者名 Koiwa, Masateru
e-Rad_Researcher 90202168 著者名 Tajima, Motoshi
著者名 Kohara, Junko
e-Rad_Researcher 40160733 著者名 Ogasawara, Satoshi
著者名 Kato, Yukinari
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
e-Rad_Researcher 10579163 著者名 Murata, Shiro
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	Bovine mycoplasmosis
	IFN-γ
	immunosuppression
	PD-1
	PD-L1
発行日	2017-09
出版者	John Wiley & Sons
収録物名	Immunity, inflammation and disease
巻(号)	5(3)
ページ	355-363
抄録	Introduction:Bovine mycoplasma, chieflyMycoplasma bovis, is a pathogen thatcauses pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogenexerts immunosuppressive effects, such as the inhibition of interferon production.However, the mechanisms involved in bovine mycoplasmosis have not been fullyelucidated. In this study, we investigated the role of the programmed death-1(PD-1)/programmed death-ligand 1 (PD-L1) pathway in immunosuppression inbovine mycoplasmosis.Methods:In the initial experiments, we used enzyme-linked immunosorbent assayto measure interferon-g(IFN-g) from peripheral blood mononuclear cells(PBMCs) isolated from cattle with mycoplasmosis.Results:Expectedly, IFN-gproduction significantly decreased in cattle withmycoplasmosis compared with that in clinically healthy cattle. Concomitantly,flow cytometric analysis revealed that the proportions of PD-1tCD4tandPD-L1tCD14tcells significantly increased in peripheral blood of the infectedcattle. Interestingly, the number of PD-1tCD4tand PD-1tCD8tT cells werenegatively correlated with IFN-gproduction from PBMCs in bovine mycoplas-mosis. Additionally, blockade of the PD-1/PD-L1 pathway in vitro by anti-bovinePD-1- and anti-bovine PD-L1 antibodies significantly upregulated the productionof IFN-gfrom anti-mycoplasma-specific cells.Conclusions:These results suggest that the PD-1/PD-L1 pathway could beinvolved in immune exhaustion of bovine mycoplasma-specific T cells. Inconclusion, our study opens up a new perspective in the therapeutic strategy forbovine mycoplasmosis by targeting the immunoinhibitory receptor pathways
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2050-4527
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1002/iid3.173