A Testis-Specific Long Non-Coding RNA, lncRNA-Tcam1, Regulates Immune-Related Genes in Mouse Male Germ Cells

Kurihara, Misuzu; Otsuka, Kai; Matsubara, Shin; Shiraishi, Akira; Satake, Honoo; Kimura, Atsushi P.

A Testis-Specific Long Non-Coding RNA, lncRNA-Tcam1, Regulates Immune-Related Genes in Mouse Male Germ Cells

Kurihara, Misuzu; Otsuka, Kai; Matsubara, Shin et al.

Frontiers in Endocrinology, 2017, 8, 299

Permalink : https://hdl.handle.net/2115/67910

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/67910
タイトル	A Testis-Specific Long Non-Coding RNA, lncRNA-Tcam1, Regulates Immune-Related Genes in Mouse Male Germ Cells
著者	Kurihara, Misuzu ; Otsuka, Kai ; Matsubara, Shin ; Shiraishi, Akira ; Satake, Honoo ; Kimura, Atsushi P.
著者名 Kurihara, Misuzu
著者名 Otsuka, Kai
著者名 Matsubara, Shin
著者名 Shiraishi, Akira
著者名 Satake, Honoo
e-Rad_Researcher 90422005 著者名 Kimura, Atsushi P.
言語	英語
キーワード	long non-coding RNA
	spermatogenesis
	GC-2spd(ts) cells
	gene activation
	immune response
発行日	2017-11-02
出版者	Frontiers Media
収録物名	Frontiers in Endocrinology
巻(号)	8
ページ	299
抄録	Spermatogenesis is precisely controlled by hormones from the hypothalamus-pituitary-gonadal axis and testis-specific genes, but the regulatory mechanism is not fully understood. Recently, a large number of long non-coding RNAs (lncRNAs) are found to be transcribed at each stage of meiosis of male germ cells, and their functions in spermatogenesis have yet to be fully investigated. lncRNA-testicular cell adhesion molecule 1 (lncRNA-Tcam1) is a nuclear lncRNA which is specifically expressed in mouse male germ cells and presumed to play a role in gene regulation during meiosis. Here, we present the identification of potential target genes of lncRNA-Tcam1 using spermatocyte-derived GC-2spd(ts) cells. Initially, 55 target gene candidates were detected by RNA-sequencing of two GC-2spd(ts) cell clones that were stably transfected with transgenes to express lncRNA-Tcam1 at different levels. Expression of 21 genes of the candidates was found to be correlated with lncRNA-Tcam1 at 7-14 postnatal days, when lncRNA-Tcam1 expression was elevated. Subsequently, we examined expression levels of the 21 genes in other two GC-2spd(ts) clones, and 11 genes exhibited the correlation with lncRNA-Tcam1. Induction of lncRNA-Tcam1 transcription using the Tet-off system verified that six genes, Trim30a, Ifit3, Tgtp2, Ifi47, Oas1g, and Gbp3, were upregulated in GC-2spd(ts) cells, indicating that lncRNA-Tcam1 is responsible for the regulation of gene expression of the six genes. In addition, five of the six genes, namely, Ifit3, Tgtp2, Ifi47, Oas1g, and Gbp3, are immune response genes, and Trim30a is a negative regulator of immune response. Altogether, the present study suggests that lncRNA-Tcam1 is responsible for gene regulation for the immune response during spermatogenesis.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1664-2392
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3389/fendo.2017.00299