How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro

Yoshimura, Yoshitaka; Kikuiri, Takashi; Hasegawa, Tomokazu; Matsuno, Mino; Minamikawa, Hajime; Deyama, Yoshiaki; Suzuki, Kuniaki

How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro

Yoshimura, Yoshitaka; Kikuiri, Takashi; Hasegawa, Tomokazu et al.

Molecular medicine reports, 2017, 16(1), 429-434

Permalink : https://hdl.handle.net/2115/68022

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アクセス権	open access
URI	https://hdl.handle.net/2115/68022
タイトル	How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro
著者	Yoshimura, Yoshitaka ; Kikuiri, Takashi ; Hasegawa, Tomokazu ; Matsuno, Mino ; Minamikawa, Hajime ; Deyama, Yoshiaki ; Suzuki, Kuniaki
e-Rad_Researcher 30230816 著者名 Yoshimura, Yoshitaka
e-Rad_Researcher 10322819 著者名 Kikuiri, Takashi
e-Rad_Researcher 50274668 著者名 Hasegawa, Tomokazu
e-Rad_Researcher 80374544 著者名 Matsuno, Mino
著者名 Minamikawa, Hajime
e-Rad_Researcher 80271667 著者名 Deyama, Yoshiaki
e-Rad_Researcher 40133748 著者名 Suzuki, Kuniaki
言語	英語
キーワード	medium volume
	mineralization
	osteoclastogenesis
	osteoblast
	osteoclast
発行日	2017-07
出版者	Spandidos Publications
収録物名	Molecular medicine reports
巻(号)	16(1)
ページ	429-434
抄録	Bone is maintained by a balance between bone formation and resorption. This remodeling is controlled by a wide variety of systemic and local factors including hormones, cytokines and mechanical stresses. The present in vitro study examined the impact of medium volume, using 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0 ml/well in a 24-well plate, on the differentiation of osteoblasts and osteoclasts. There were no differences in the alkaline phosphatase activity of osteoblasts amongst the groups; however, the area of mineral deposition was decreased in a media volume-dependent manner. A co-culture of osteoblastic cells with bone marrow cells revealed a reduction in the total number of osteoclastic tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (>= 2 nuclei), whereas the formation of large osteoclastic TRAP-positive multinuclear cells (>= 8 nuclei) was increased, in a media volume-dependent manner. There were also no differences in receptor activator of nuclear factor-kappa B ligand mRNA and total osteoprotegerin (OPG) protein expression levels amongst the groups, however the concentration of OPG decreased in a media volume-dependent manner. In conclusion, the present study demonstrated that the suppression of mineralization in osteoblastic cells and the stimulation of osteoclast fusion are dependent on the medium volume, indicating that media volume is an important factor in in vitro cell culture systems.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1791-2997
関連情報	PMID 28535008
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3892/mmr.2017.6611