open access

https://hdl.handle.net/2115/68347

Expression and in situ localization ofGATA4, 5and6 mRNAs in ovine conceptuses and uterine endometria during the peri-implantation period

英語

peri-implantation period

2014-04

Blackwell Publishing

Animal Science Journal

85(4)

388-394

Abstract: In vertebrates, six GATA transcription factors, GATA1 through GATA6, have been identified and GATA1-3 is known to be involved in hematopoietic developments, while GATA4-6 play roles in cardiac and endoderm developments. Recently, we and others have found that GATA2 and GATA3 found in the trophectoderm plays a role in gene expression specific to this cell type, but GATA4-6 have not been well characterized in early embryonic developments. Using quantitative polymerase chain reaction (qPCR) and in situ hybridization, we examined the expression of GATA4, 5 and 6 messenger RNAs (mRNAs) in ovine conceptuses and uteri during the peri-implantation period. In ovine conceptuses, GATA4, 5 and 6 transcripts were present on days 15, 17 and 21 (day 0 = day of mating), and high GATA5 and 6 mRNAs were found on day 21, most of which were localized in the trophectoderm and endoderm. Moreover, minute and substantial GATA4 and 5 mRNAs were found in days 15 and 21 uterine endometria, respectively. Increase in GATA4-6 transcripts in day 21 uteri indicates that in addition to GATA1-3, GATA4-6 may also play a potentially novel role in the development of ovine trophectoderm, endoderm and/or uterine endometria following conceptus attachment to the uterine epithelium.

学術雑誌論文

Accepted Manuscript

© 2013 Japanese Society of Animal Science, This is the peer reviewed version of the following article: [Animal Science Journal; 85(4): pp388-394, 2014], which has been published in final form at [10.1111/asj.12156]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

1344-3941

1740-0929