Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate

Sakuma, Chisato; Sekizuka, Tsuyoshi; Kuroda, Makoto; Kasai, Fumio; Saito, Kyoko; Ikeda, Masaki; Yamaji, Toshiyuki; Osada, Naoki; Hanada, Kentaro

Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate

Sakuma, Chisato; Sekizuka, Tsuyoshi; Kuroda, Makoto et al.

Scientific reports, 2018, 8, 644

Permalink : https://hdl.handle.net/2115/68378

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アクセス権	open access
URI	https://hdl.handle.net/2115/68378
タイトル	Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate
著者	Sakuma, Chisato ; Sekizuka, Tsuyoshi ; Kuroda, Makoto ; Kasai, Fumio ; Saito, Kyoko ; Ikeda, Masaki ; Yamaji, Toshiyuki ; Osada, Naoki ; Hanada, Kentaro
著者名 Sakuma, Chisato
著者名 Sekizuka, Tsuyoshi
著者名 Kuroda, Makoto
著者名 Kasai, Fumio
著者名 Saito, Kyoko
著者名 Ikeda, Masaki
著者名 Yamaji, Toshiyuki
e-Rad_Researcher 70416270 著者名 Osada, Naoki
著者名 Hanada, Kentaro
言語	英語
発行日	2018-01-12
出版者	Nature Publishing Group
収録物名	Scientific reports
巻(号)	8
ページ	644
抄録	African green monkey (AGM)-derived Vero cells have been utilized to produce various human vaccines. The Vero cell genome harbors a variety of simian endogenous type D retrovirus (SERV) sequences. In this study, a transcriptome analysis showed that DNA hypomethylation released the epigenetic repression of SERVs in Vero cells. Moreover, comparative genomic analysis of three Vero cell sublines and an AGM reference revealed that the genomes of the sublines have similar to 80 SERV integrations. Among them, similar to 60 integrations are present within all three cell sublines and absent from the reference sequence. At least several of these integrations consist of complete SERV proviruses. These results strongly suggest that SERVs integrated in the genome of Vero cells did not retrotranspose after the establishment of the cell lineage as far as cells were maintained under standard culture and passage conditions, providing a scientific basis for controlling the quality of pharmaceutical cell substrates and their derived biologics.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2045-2322
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/s41598-017-18934-2