First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt

Diab, Hassan Mahmoud; Nakajima, Chie; Kotb, Saber A.; Mokhtar, Alaa; Khder, Nagwa F.M.; Abdelaal, Ahmed S.A.; Hegazy, Azza; Poudel, Ajay; Shah, Yogendra; Suzuki, Yasuhiko

First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt

Diab, Hassan Mahmoud; Nakajima, Chie; Kotb, Saber A. et al.

Tuberculosis, 2016, 96, 13-20

Permalink : https://hdl.handle.net/2115/68587

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/68587
タイトル	First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt
著者	Diab, Hassan Mahmoud ; Nakajima, Chie ; Kotb, Saber A. ; Mokhtar, Alaa ; Khder, Nagwa F.M. ; Abdelaal, Ahmed S.A. ; Hegazy, Azza ; Poudel, Ajay ; Shah, Yogendra ; Suzuki, Yasuhiko
著者名 Diab, Hassan Mahmoud
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Kotb, Saber A.
著者名 Mokhtar, Alaa
著者名 Khder, Nagwa F.M.
著者名 Abdelaal, Ahmed S.A.
著者名 Hegazy, Azza
著者名 Poudel, Ajay
著者名 Shah, Yogendra
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Genotyping
	MTB lineages
	Egypt
発行日	2016-01
出版者	Elsevier
収録物名	Tuberculosis
巻(号)	96
ページ	13-20
抄録	The present study aimed to assess the population structure of Mycobacterium tuberculosis (MTB) isolates from Egypt. A total of 230 MTB isolates were analysed using spoligotyping, large sequence polymorphism (LSPs), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and multi-locus sequence typing (MLST). The majority of isolates (93.0%) belonged to lineage 4, including 44.3, 13.4 and 10.8% of the ill-defined T clade, LAM and Haarlem families, respectively, and lineage 3 was identified in 7.0% of the isolates. MIRU-VNTRs typing allowed efficient discrimination of the spoligotype-defined clusters, including spoligo-international types (SIT) 53, 34, and 4, into 56 patterns, including 13 clusters and 43 unique patterns. A new SNP at position 311614 was identified in all six isolates to form the biggest MIRU-VNTR cluster, which suggested a recent clonal expansion. This SNP could possibly be used as a genetic marker for robust discriminations of Egyptian MTB isolates belonging to SIT53. The combination of spoligotyping, 12 MIRU-VNTRs loci and MLST provided insight into the genetic diversity and transmission dynamics of the Egyptian MTB genotypes and could be a key to implementation of effective control measures by public health authorities.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2015, Elsevier. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1472-9792
NCID	AA11658683
関連情報	PMID 26786649
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tube.2015.11.002