Altered balance of epidermis-related chemokines in epidermolysis bullosa

Ujiie, Inkin; Fujita, Yasuyuki; Nakayama, Chihiro; Matsumura, Wakana; Suzuki, Shotaro; Shinkuma, Satoru; Nomura, Toshifumi; Abe, Riichiro; Shimizu, Hiroshi

Altered balance of epidermis-related chemokines in epidermolysis bullosa

Ujiie, Inkin; Fujita, Yasuyuki; Nakayama, Chihiro et al.

Journal of dermatological science, 2017, 86(1), 37-45

Permalink : https://hdl.handle.net/2115/68661

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/68661
タイトル	Altered balance of epidermis-related chemokines in epidermolysis bullosa
著者	Ujiie, Inkin ; Fujita, Yasuyuki ; Nakayama, Chihiro ; Matsumura, Wakana ; Suzuki, Shotaro ; Shinkuma, Satoru ; Nomura, Toshifumi ; Abe, Riichiro ; Shimizu, Hiroshi
著者名 Ujiie, Inkin
著者名 Fujita, Yasuyuki
著者名 Nakayama, Chihiro
著者名 Matsumura, Wakana
著者名 Suzuki, Shotaro
e-Rad_Researcher 00613788 著者名 Shinkuma, Satoru
著者名 Nomura, Toshifumi
e-Rad_Researcher 60344511 著者名 Abe, Riichiro
e-Rad_Researcher 00146672 著者名 Shimizu, Hiroshi
言語	英語
キーワード	Epidermolysis bullosa
	Chemokine
	CCL27
	HMGB1
	SDF-1
発行日	2017-04
出版者	Elsevier
収録物名	Journal of dermatological science
巻(号)	86(1)
ページ	37-45
抄録	Background: Epidennolysis bullosa (EB) is a congenital, refractory skin disease and there are no fundamental treatments. Recently, allogenic cell therapies are beginning to be applied as potential treatments, that are based on the concept that the allogenic cells can migrate into the skin and reconstitute the skin components. Although the mechanisms of cell migration into skin are not fully understood, chemokines are regarded as key factors in recruiting bone marrow-derived cells. Objectives: Our study aims to elucidate the expression of chemokines in the EB patients. Methods: We determined the expression of wound-healing related chemokines in the sera, keratinocytes, and skin tissues of EB patients and compared them to those of healthy volunteers by enzyme-linked immunosorbent assays, quantitative reverse transcription PCR, and immunofluorescence staining. Results: The serum levels of CXCL12 and HMGB1 were found to be significantly elevated in the EB patients. Conversely, the serum levels of CCL21 were found to be lower in the EB patients than in healthy controls. In addition, the serum levels of CXCL12 tended to increase and the serum levels of CCL27 tended to decrease with an increase in the affected body surface areas. To detect the origin of the circulating chemokines, we performed immunofluorescence staining. CCL21, CCL27, HMGB1 and CXCL12 were stained more broadly in the EB patient tissues than those in the control tissues. Conclusions: These results suggest that fluctuations in chemokine levels may contribute in a coordinated way to the wound-healing process and lend clues toward efficient cell therapies for EB.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0923-1811
関連情報	PMID 28094098
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jdermsci.2016.12.021