Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii

Pratiwi, Putri; Tanaka, Genta; Takahashi, Tomohiro; Xie, Xiaonan; Yoneyama, Koichi; Matsuura, Hideyuki; Takahashi, Kosaku

Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii

Pratiwi, Putri; Tanaka, Genta; Takahashi, Tomohiro et al.

Plant and Cell Physiology, 2017, 58(4), 789-801

Permalink : https://hdl.handle.net/2115/68680

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アクセス権	open access
URI	https://hdl.handle.net/2115/68680
タイトル	Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii
著者	Pratiwi, Putri ; Tanaka, Genta ; Takahashi, Tomohiro ; Xie, Xiaonan ; Yoneyama, Koichi ; Matsuura, Hideyuki ; Takahashi, Kosaku
著者名 Pratiwi, Putri
著者名 Tanaka, Genta
著者名 Takahashi, Tomohiro
著者名 Xie, Xiaonan
著者名 Yoneyama, Koichi
e-Rad_Researcher 20344492 著者名 Matsuura, Hideyuki
e-Rad_Researcher 30374622 著者名 Takahashi, Kosaku
言語	英語
キーワード	AOC
	AOS
	JAR1
	Jasmonic acid
	OPR3
	Selaginella moellendorffii
発行日	2017-04
出版者	Oxford University Press
収録物名	Plant and Cell Physiology
巻(号)	58(4)
ページ	789-801
抄録	Jasmonic acid (JA) is involved in a variety of physiological responses in seed plants. However, the detection and role of JA in lycophytes, a group of seedless vascular plants, have remained elusive until recently. This study provides the first evidence of 12-oxo-phytodienoic acid (OPDA), JA and jasmonoyl-isoleucine (JA-Ile) in the model lycophyte Selaginella moellendorffii. Mechanical wounding stimulated the accumulation of OPDA, JA and JA-Ile. These data were corroborated by the detection of enzymatically active allene oxide synthase (AOS), allene oxide cyclase (AOC), 12-oxo-phytodienoic acid reductase 3 (OPR3) and JA-Ile synthase (JAR1) in S. moellendorffii. SmAOS2 is involved in the first committed step of JA biosynthesis. SmAOC1 is a crucial enzyme for generating the basic structure of jasmonates and is actively involved in the formation of OPDA. SmOPR5, a functionally active OPR3-like enzyme, is also vital for the reduction of (+)-cis-OPDA, the only isomer of the JA precursor. The conjugation of JA to Ile by SmJAR1 demonstrates that S. moellendorffii produces JA-Ile. Thus, the four active enzymes have characteristics similar to those in seed plants. Wounding and JA treatment induced the expression of SmAOC1 and SmOPR5. Furthermore, JA inhibited the growth of shoots in S. moellendorffii, which suggests that JA functions as a signaling molecule in S. moellendorffii. This study proposes that JA evolved as a plant hormone for stress adaptation, beginning with the emergence of vascular plants.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant & cell physiology following peer review. The version of record 58(4), pp789-801 is available online at: https://doi.org/10.1093/pcp/pcx031
PISSN	0032-0781
EISSN	1471-9053
NCID	AA0077511X
関連情報	PMID 28340155
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/pcp/pcx031