Heme Iron Coordination Structure of Heme Transport Protein HutB from Vibrio Cholerae

Uchida, Takeshi; Funamizu, Takumi; Ogura, Mariko et al.

Bulletin of the Chemical Society of Japan, 2017, 90(8), 924-930

Permalink : https://hdl.handle.net/2115/68744

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アクセス権	open access
URI	https://hdl.handle.net/2115/68744
タイトル	Heme Iron Coordination Structure of Heme Transport Protein HutB from Vibrio Cholerae
著者	Uchida, Takeshi ; Funamizu, Takumi ; Ogura, Mariko ; Ishimori, Koichiro
e-Rad_Researcher 30343742 著者名 Uchida, Takeshi
著者名 Funamizu, Takumi
著者名 Ogura, Mariko
e-Rad_Researcher 20192487 著者名 Ishimori, Koichiro
言語	英語
発行日	2017-08
出版者	Chemical Society of Japan
収録物名	Bulletin of the Chemical Society of Japan
巻(号)	90(8)
ページ	924-930
抄録	HutB is a putative heme transport protein located in the periplasmic space in Vibrio cholerae. Here, we purified HutB and characterized its heme binding properties. An analysis of the Soret band showed that there are two types of heme binding geometries depending on the heme concentration: 404-nm species are dominant at lower concentrations of heme, and 394-nm species dominate at higher concentrations. Moreover, a mutational study revealed that either Tyr65 or Tyr198 binds heme with the help of histidine, a property shared with another V. cholerae heme transport protein, HutX, despite the absence of sequence similarity, indicating that HutB acts as a heme transport protein in the periplasm.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0009-2673
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1246/bcsj.20170104