Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane

Amarasiri, Mohan; Hashiba, Satoshi; Miura, Takayuki; Nakagomi, Toyoko; Nakagomi, Osamu; Ishii, Satoshi; Okabe, Satoshi; Sano, Daisuke

Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane

Amarasiri, Mohan; Hashiba, Satoshi; Miura, Takayuki et al.

Water research, 2016, 95, 383-391

Permalink : https://hdl.handle.net/2115/70214

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アクセス権	open access
URI	https://hdl.handle.net/2115/70214
タイトル	Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane
著者	Amarasiri, Mohan ; Hashiba, Satoshi ; Miura, Takayuki ; Nakagomi, Toyoko ; Nakagomi, Osamu ; Ishii, Satoshi ; Okabe, Satoshi ; Sano, Daisuke
著者名 Amarasiri, Mohan
著者名 Hashiba, Satoshi
著者名 Miura, Takayuki
e-Rad_Researcher 40155693 著者名 Nakagomi, Toyoko
e-Rad_Researcher 70143047 著者名 Nakagomi, Osamu
著者名 Ishii, Satoshi
e-Rad_Researcher 10253816 著者名 Okabe, Satoshi
e-Rad_Researcher 80550368 著者名 Sano, Daisuke
言語	英語
キーワード	Extracellular polymeric substance
	Histo-blood group antigen
	Human norovirus
	Lipopolysaccharide
	Microfiltration
	Membrane bioreactor
発行日	2016-05-15
出版者	Elsevier
収録物名	Water research
巻(号)	95
ページ	383-391
抄録	We demonstrated the genotype-dependent removal of human norovirus particles with a microfiltration (MF) membrane in the presence of bacteria bearing histo-blood group antigens (HBGAs). Three genotypes (GII.3, GII.4, and GII.6) of norovirus-like particles (NoVLPs) were mixed with three bacterial strains (Enterobacter sp. SENG-6, Escherichia coli 086:K61:B7, and Staphylococcus epidermidis), respectively, and the mixture was filtered with an MF membrane having a nominal pore size of 0.45 mu m. All NoVLP genotypes were rejected by the MF membrane in the presence of Enterobacter sp. SENG-6, which excreted HBGAs as extracellular polymeric substances (EPS). This MF membrane removal of NoVLPs was not significant when EPS was removed from cells of Enterobacter sp. SENG-6. GII.6 NoVLP was not rejected with the MF membrane in the presence of E. coli 086:K61:B7, but the removal of EPS of E. coli 086:K61:B7 increased the removal efficiency due to the interaction of NoVLPs with the exposed B-antigen in lipopolysaccharide (LPS) of E. coli 086:K61:B7. No MF membrane removal of all three genotypes was observed when S. epidermidis, an HBGA-negative strain, was mixed with NoVLPs. These results demonstrate that the location of HBGAs on bacterial cells is an important factor in determining the genotype-dependent removal efficiency of norovirus particles with the MF membrane. The presence of HBGAs in mixed liquor suspended solids from a membrane bioreactor (MBR) pilot plant was confirmed by immune-transmission electron microscopy, which implies that bacterial HBGAs can contribute to the genotype-dependent removal of human noroviruses with MBR using MF membrane.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0043-1354
関連情報	PMID 27095709
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.watres.2016.04.018