Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging

Kubota, Harue; Komarov, Denis A.; Yasui, Hironobu; Matsumoto, Shingo; Inanami, Osamu; Kirilyuk, Igor A.; Khramtsov, Valery V.; Hirata, Hiroshi

Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging

Kubota, Harue; Komarov, Denis A.; Yasui, Hironobu et al.

Magnetic Resonance Materials in Physics, Biology and Medicine, 2017, 30(3), 291-298

Permalink : https://hdl.handle.net/2115/70631

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アクセス権	open access
URI	https://hdl.handle.net/2115/70631
タイトル	Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging
著者	Kubota, Harue ; Komarov, Denis A. ; Yasui, Hironobu ; Matsumoto, Shingo ; Inanami, Osamu ; Kirilyuk, Igor A. ; Khramtsov, Valery V. ; Hirata, Hiroshi
著者名 Kubota, Harue
著者名 Komarov, Denis A.
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
著者名 Matsumoto, Shingo
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
著者名 Kirilyuk, Igor A.
著者名 Khramtsov, Valery V.
e-Rad_Researcher 60250958 著者名 Hirata, Hiroshi
言語	英語
キーワード	Nitroxyl radical
	Clonogenic assay
	In vivo nitroxyl radical kinetics
	In vivo EPR
	T-2* mapping
	Single-point imaging
発行日	2017-06
出版者	Springer
収録物名	Magnetic Resonance Materials in Physics, Biology and Medicine
巻(号)	30(3)
ページ	291-298
抄録	Objectives The aim of this study was to demonstrate the feasibility of in vivo three-dimensional (3D) relaxation time T-2* mapping of a dicarboxy-PROXYL radical using continuous-wave electron paramagnetic resonance (CW-EPR) imaging. Materials and methods Isotopically substituted dicarboxy-PROXYL radicals, 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-( 3,4-H-2(2))-(1-N-15)-1-oxyl (H-2, N-15-DCP) and 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-(3,4-H-2(2))1- oxyl (H-2-DCP), were used in the study. A clonogenic cell survival assay was performed with the H-2-DCP radical using squamous cell carcinoma (SCC VII) cells. The time course of EPR signal intensities of intravenously injected H-2, N-15-DCP and H-2-DCP radicals were determined in tumor-bearing hind legs of mice (C3H/HeJ, male, n = 5). CW-EPR-based single-point imaging (SPI) was performed for 3D T-2* mapping. Results H-2-DCP radical did not exhibit cytotoxicity at concentrations below 10 mM. The in vivo half-life of H-2, N-15-DCP in tumor tissues was 24.7 +/- 2.9 min (mean +/- standard deviation [SD], n = 5). The in vivo time course of the EPR signal intensity of the H-2, N-15-DCP radical showed a plateau of 10.2 +/- 1.2 min (mean +/- SD) where the EPR signal intensity remained at more than 90% of the maximum intensity. During the plateau, in vivo 3D T-2* maps with H-2, N-15-DCP were obtained from tumor-bearing hind legs, with a total acquisition time of 7.5 min. Conclusion EPR signals of H-2, N-15-DCP persisted long enough after bolus intravenous injection to conduct in vivo 3D T-2* mapping with CW-EPR-based SPI.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	"The original publication is available at www.springerlink.com".
PISSN	0968-5243
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10334-016-0606-8