Genetic polymorphisms in Plasmodium falciparum chloroquine resistance genes, pfcrt and pfmdr1, in North Sulawesi, Indonesia

Reteng, Patrick; Vrisca, Visia; Sukarno, Inka; Djarkoni, Ilham Habib; Kalangi, Jane Angela; Jacobs, George Eduardo; Runtuwene, Lucky Ronald; Eshita, Yuki; Maeda, Ryuichiro; Suzuki, Yutaka; Mongan, Arthur Elia; Warouw, Sarah Maria; Yamagishi, Junya; Tuda, Josef

Genetic polymorphisms in Plasmodium falciparum chloroquine resistance genes, pfcrt and pfmdr1, in North Sulawesi, Indonesia

Reteng, Patrick; Vrisca, Visia; Sukarno, Inka et al.

BMC Research Notes, 2017, 10, 147

Permalink : https://hdl.handle.net/2115/70696

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アクセス権	open access
URI	https://hdl.handle.net/2115/70696
タイトル	Genetic polymorphisms in Plasmodium falciparum chloroquine resistance genes, pfcrt and pfmdr1, in North Sulawesi, Indonesia
著者	Reteng, Patrick ; Vrisca, Visia ; Sukarno, Inka ; Djarkoni, Ilham Habib ; Kalangi, Jane Angela ; Jacobs, George Eduardo ; Runtuwene, Lucky Ronald ; Eshita, Yuki ; Maeda, Ryuichiro ; Suzuki, Yutaka ; Mongan, Arthur Elia ; Warouw, Sarah Maria ; Yamagishi, Junya ; Tuda, Josef
著者名 Reteng, Patrick
著者名 Vrisca, Visia
著者名 Sukarno, Inka
著者名 Djarkoni, Ilham Habib
著者名 Kalangi, Jane Angela
著者名 Jacobs, George Eduardo
著者名 Runtuwene, Lucky Ronald
e-Rad_Researcher 10082223 著者名 Eshita, Yuki
著者名 Maeda, Ryuichiro
e-Rad_Researcher 40323646 著者名 Suzuki, Yutaka
著者名 Mongan, Arthur Elia
著者名 Warouw, Sarah Maria
e-Rad_Researcher 80535328 著者名 Yamagishi, Junya
著者名 Tuda, Josef
言語	英語
キーワード	Chloroquine
	Genetic polymorphism
	Indonesia
	Malaria
	Multiplex sequencing
	North Sulawesi
	PCR–RFLP
	pfcrt
	pfmdr1
	Plasmodium falciparum
発行日	2017-04-04
出版者	BMC
収録物名	BMC Research Notes
巻(号)	10
ページ	147
抄録	[Background] Malaria still poses one of the major threats to human health. Development of effective antimalarial drugs has decreased this threat; however, the emergence of drug-resistant Plasmodium falciparum, a cause of Malaria, is disconcerting. The antimalarial drug chloroquine has been effectively used, but resistant parasites have spread worldwide. Interestingly, the withdrawal of the drug reportedly leads to an increased population of susceptible parasites in some cases. We examined the prevalence of genomic polymorphisms in a malaria parasite P. falciparum, associated with resistance to an antimalarial drug chloroquine, after the withdrawal of the drug from Indonesia. [Results] Blood samples were collected from 95 malaria patients in North Sulawesi, Indonesia, in 2010. Parasite DNA was extracted and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for pfcrt and pfmdr1. In parallel, multiplex amplicon sequencing for the same genes was carried out with Illumina MiSeq. Of the 59 cases diagnosed as P. falciparum infection by microscopy, PCR-RFLP analysis clearly identified the genotype 76T in pfcrt in 44 cases. Sequencing analysis validated the identified genotypes in the 44 cases and demonstrated that the haplotype in the surrounding genomic region was exclusively SVMNT. Results of pfmdr1 were successfully obtained for 51 samples, where the genotyping results obtained by the two methods were completely consistent. In pfmdr1, the 86Y mutant genotype was observed in 45 cases (88.2%). [Conclusions] Our results suggest that the prevalence of the mutated genotypes remained dominant even 6 years after the withdrawal of chloroquine from this region. Diversified haplotype of the resistance-related locus, potentially involved in fitness costs, unauthorized usage of chloroquine, and/or a short post-withdrawal period may account for the observed high persistence of prevalence.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1756-0500
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s13104-017-2468-1