Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke

Miyamoto, Michiyuki; Nakamura, Kentaro; Shichinohe, Hideo; Yamauchi, Tomohiro; Ito, Masaki; Saito, Hisayasu; Kawabori, Masahito; Osanai, Toshiya; Sasaki, Tasuku; Houkin, Kiyohiro; Kuroda, Satoshi

Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke

Miyamoto, Michiyuki; Nakamura, Kentaro; Shichinohe, Hideo et al.

Stem Cells International, 2018, 2018, 4829534

Permalink : https://hdl.handle.net/2115/70782

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アクセス権	open access
URI	https://hdl.handle.net/2115/70782
タイトル	Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke
著者	Miyamoto, Michiyuki ; Nakamura, Kentaro ; Shichinohe, Hideo ; Yamauchi, Tomohiro ; Ito, Masaki ; Saito, Hisayasu ; Kawabori, Masahito ; Osanai, Toshiya ; Sasaki, Tasuku ; Houkin, Kiyohiro ; Kuroda, Satoshi
著者名 Miyamoto, Michiyuki
著者名 Nakamura, Kentaro
e-Rad_Researcher 80374479 著者名 Shichinohe, Hideo
著者名 Yamauchi, Tomohiro
著者名 Ito, Masaki
著者名 Saito, Hisayasu
e-Rad_Researcher 50399870 著者名 Kawabori, Masahito
e-Rad_Researcher 90622788 著者名 Osanai, Toshiya
著者名 Sasaki, Tasuku
e-Rad_Researcher 90229146 著者名 Houkin, Kiyohiro
e-Rad_Researcher 10301904 著者名 Kuroda, Satoshi
言語	英語
発行日	2018-04-08
出版者	Hindawi
収録物名	Stem Cells International
巻(号)	2018
ページ	4829534
抄録	Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo). A BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex 7 days after MCAo. A BMSC suspension or vehicle was transplanted into the ipsilateral striatum. Rat motor function was serially evaluated and histological analysis was performed 5 weeks after transplantation. The results showed that BMSCs could proliferate well in the RCP sponge and the BMSC-RCP sponge significantly promoted functional recovery, compared with the vehicle group. Histological analysis revealed that the RCP sponge provoked few inflammatory reactions in the host brain. Moreover, some BMSCs migrated to the peri-infarct area and differentiated into neurons in the BMSC-RCP sponge group. These findings suggest that the RCP sponge may be a promising candidate for animal protein-free scaffolds in cell therapy for ischemic stroke in humans.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1687-966X
EISSN	1687-9678
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1155/2018/4829534