open access

https://hdl.handle.net/2115/70898

Cytopathologic findings of cell block materials from the vitreous : Diagnostic distinction between intraocular lymphoma and non-lymphomatous diseases

英語

vitreous body

2017-07

John Wiley & Sons

Pathology international

67(7)

342-349

Intraocular lymphoma is a rare neoplasm that occurs only in the eyes and/or central nervous system. Diagnosis of intraocular lymphoma is difficult because its clinical manifestations mimic chronic uveitis. Pathological examination of the vitreous is one of the main diagnostic tools for intraocular lymphoma, but this is challenging due to the sparse cellularity and specimen degeneration. Here, we reviewed 33 cell block preparations from vitreous perfusion fluid in order to examine the significance of cytopathological findings for differential diagnosis using vitreous samples. The cases comprised 12 intraocular lymphomas and 21 non-lymphomatous diseases. Cytologically, vitreous samples from non-lymphoma cases showed lower cellularity than the lymphoma cases. Whereas vitreous material from cases with infectious endophthalmitis showed prominent neutrophilic infiltration, material from sarcoidosis cases showed infiltration of small lymphoid cells, especially CD4-positive T cells. On the other hand, lymphoma cases showed higher cellularity, with large, irregular and atypical lymphoid cells, frequent necrotic cells in the background, and less pronounced neutrophil infiltration. Immunocytochemically, 11 of the 12 lymphoma cases were of the B-cell phenotype and the remaining case was of the T/NK-cell phenotype. In conclusion, careful cytopathological examination or immunocytochemistry of vitreous material facilitates appropriate diagnosis of intraocular lymphoma.

学術雑誌論文

Accepted Manuscript

This is the pre-peer reviewed version of the following article: Hiromi Kanno-Okada, Emi Takakuwa, Yoshiaki Tagawa, Satoru Kase, Kanako C. Hatanaka, Yutaka Hatanaka, Kenichi Namba, Tomoko Mitsuhashi, Yoshihiro Matsuno. Cytopathologic findings of cell block materials from the vitreous : Diagnostic distinction between intraocular lymphoma and non‐lymphomatous diseases. Pathology International, Volume 67, Issue 7, pp.342-349, 2017, which has been published in final form at https://doi.org/10.1111/pin.12551. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

1320-5463