Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Abdel-Ghani, Mohammed. A.; Sakaguchi, Kenichiro; Kanno, Chihiro; Yanagawa, Yojiro; Katagiri, Seiji; Nagano, Masashi

Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

Abdel-Ghani, Mohammed. A.; Sakaguchi, Kenichiro; Kanno, Chihiro et al.

Journal of Reproduction and Development, 2018, 64(4), 365-369

Permalink : https://hdl.handle.net/2115/71093

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アクセス権	open access
URI	https://hdl.handle.net/2115/71093
タイトル	Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes
著者	Abdel-Ghani, Mohammed. A. ; Sakaguchi, Kenichiro ; Kanno, Chihiro ; Yanagawa, Yojiro ; Katagiri, Seiji ; Nagano, Masashi
著者名 Abdel-Ghani, Mohammed. A.
著者名 Sakaguchi, Kenichiro
著者名 Kanno, Chihiro
著者名 Yanagawa, Yojiro
e-Rad_Researcher 00292061 著者名 Katagiri, Seiji
e-Rad_Researcher 70312402 著者名 Nagano, Masashi
言語	英語
キーワード	IBMX
	Oocytes
	Pre-IVM duration
発行日	2018-05-24
出版者	日本繁殖生物学会
収録物名	Journal of Reproduction and Development
巻(号)	64(4)
ページ	365-369
記述	Advance Publication
抄録	We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subjected to in vitro maturation, fertilization, and culture. The cleavage rate (73%) of small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (61 and 62%, respectively). The blastocyst rate (16%) of embryos derived from small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (9 and 8%, respectively). In addition, small-sized oocytes with 5 h pre-IVM had a higher mean cell number in blastocysts (134.1 ± 34.8) than those with 0 and 10 h pre-IVM (100.2 ± 17.2 and 107.8 ± 23.7, respectively). In conclusion, the pre-IVM of small-sized oocytes with IBMX for 5 h improved the developmental competence of bovine oocytes, as well as the quality of blastocysts.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-8818
EISSN	1348-4400
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2018-004