Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus nippon yesoensis), Japan

Elbaz, Elzahara; Moustafa, Mohamed Abdallah Mohamed; Lee, Kyunglee; Mohamed, Wessam Mohamed Ahmed; Nakao, Ryo; Shimozuru, Michito; Sashika, Mariko; Younis, Emad Elsayed Ahmed; El-Khodery, Sabry Ahmed; Tsubota, Toshio

Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus nippon yesoensis), Japan

Elbaz, Elzahara; Moustafa, Mohamed Abdallah Mohamed; Lee, Kyunglee et al.

Ticks and Tick-Borne Diseases, 2017, 8(5), 802-807

Permalink : https://hdl.handle.net/2115/71113

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アクセス権	open access
URI	https://hdl.handle.net/2115/71113
タイトル	Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus nippon yesoensis), Japan
著者	Elbaz, Elzahara ; Moustafa, Mohamed Abdallah Mohamed ; Lee, Kyunglee ; Mohamed, Wessam Mohamed Ahmed ; Nakao, Ryo ; Shimozuru, Michito ; Sashika, Mariko ; Younis, Emad Elsayed Ahmed ; El-Khodery, Sabry Ahmed ; Tsubota, Toshio
著者名 Elbaz, Elzahara
著者名 Moustafa, Mohamed Abdallah Mohamed
著者名 Lee, Kyunglee
著者名 Mohamed, Wessam Mohamed Ahmed
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
著者名 Shimozuru, Michito
著者名 Sashika, Mariko
著者名 Younis, Emad Elsayed Ahmed
著者名 El-Khodery, Sabry Ahmed
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
言語	英語
キーワード	Babesia
	Theileria
	Sika deer
	Reverse line blot
発行日	2017-08
出版者	Elsevier
収録物名	Ticks and Tick-Borne Diseases
巻(号)	8(5)
ページ	802-807
抄録	Babesia and Theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesia and Theileria species by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87.7% (236/269) and 11.5% (31/269) of the examined PCR products hybridized with the probes which were designed to detect all Babesia and Theileria spp., all Theileria spp., all Babesia spp., Theileria sp. Thrivae and Babesia divergens-like, respectively. The 18S rRNA gene partial sequences were divided into Theileria sp. Thrivae, T. capreoli, B. divergens-like and an undescribed Babesia species. This study showed the first detection of the undescribed Babesia sp. from Japan. Therefore, more studies are required to understand the ecology of the newly detected tick-borne pathogens in Hokkaido.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1877-959X
関連情報	PMID 28651947
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ttbdis.2017.06.007