Diversity changes of microbial communities into hospital surface environments

Yano, Rika; Shimoda, Tomoko; Watanabe, Reina; Kuroki, Yasutoshi; Okubo, Torahiko; Nakamura, Shinji; Matsuo, Junji; Yoshimura, Sadako; Yamaguchi, Hiroyuki

Diversity changes of microbial communities into hospital surface environments

Yano, Rika; Shimoda, Tomoko; Watanabe, Reina et al.

Journal of Infection and Chemotherapy, 2017, 23(7), 439-445

Permalink : https://hdl.handle.net/2115/71154

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アクセス権	open access
URI	https://hdl.handle.net/2115/71154
タイトル	Diversity changes of microbial communities into hospital surface environments
著者	Yano, Rika ; Shimoda, Tomoko ; Watanabe, Reina ; Kuroki, Yasutoshi ; Okubo, Torahiko ; Nakamura, Shinji ; Matsuo, Junji ; Yoshimura, Sadako ; Yamaguchi, Hiroyuki
e-Rad_Researcher 50250519 著者名 Yano, Rika
e-Rad_Researcher 60576180 著者名 Shimoda, Tomoko
e-Rad_Researcher 10431313 著者名 Watanabe, Reina
著者名 Kuroki, Yasutoshi
著者名 Okubo, Torahiko
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
e-Rad_Researcher 50359486 著者名 Matsuo, Junji
e-Rad_Researcher 10182817 著者名 Yoshimura, Sadako
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	Hygiene
	Nosocomial infection
	Hospital cleanliness
	16S rRNA sequence analysis
	Microbiome
発行日	2017-07
出版者	Elsevier
収録物名	Journal of Infection and Chemotherapy
巻(号)	23(7)
ページ	439-445
抄録	Previous works have demonstrated considerable variability in hospital cleanliness in Japan, suggesting that contamination is driven by factors that are currently poorly controlled. We undertook 16S rRNA sequence analysis to study population structures of hospital environmental microbiomes to see which factor(s) impacted contamination. One hundred forty-four samples were collected from surfaces of three hospitals with distinct sizes (“A”: >500 beds, “B”: 100–500 beds, “C”: <100 beds). Sample locations of two ward types (Surgical and Internal) included patient room bed table (multiple) (4BT), patient overbed table (multiple) (4OT), patient room sink (multiple) (4S), patient room bed table (single) (SBT), patient overbed table (single) (SOT), patient room sink (single) (SS), nurse desk (ND), and nurse wagon (NW). Total DNA was extracted from each sample, and the 50 samples that yielded sufficient DNA were used for further 16S rRNA sequencing of hospital microbiome populations with cluster analysis. The number of assigned bacterial OTU populations was significantly decreased in hospital “C” compared to the other hospitals. Cluster analysis of sampling locations revealed that the population structure in almost all locations of hospital “C” and some locations in the other hospitals was very similar and unusually skewed with a family, Enterobacteriaceae. Interestingly, locations included patient area (4OT, 4BT, SBT) and nurse area (ND), with a device (NW) bridging the two and a place (4S and SS) shared between patients or visitors. We demonstrated diversity changes of hospital environmental microbiomes with a skewed population, presumably by medical staff pushing NWs or sinks shared by patients or visitors.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1341-321X
EISSN	1437-7780
関連情報	PMID 28431935
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jiac.2017.03.016