open access

https://hdl.handle.net/2115/71308

Dietary alpha-cyclodextrin reduces atherosclerosis and modifies gut flora in apolipoprotein E-deficient mice

英語

Gut flora

2017-08-16

John Wiley & Sons

Molecular nutrition & food research

61(8)

1600804

Scope: alpha-Cyclodextrin (alpha-CD), a cyclic polymer of glucose, has been shown to lower plasma cholesterol in animals and humans; however, its effect on atherosclerosis has not been previously described. Methods and results: apoE-knockout mice were fed either low-fat diet (LFD; 5.2% fat, w/w), or Western high fat diet (21.2% fat) containing either no additions (WD), 1.5% alpha-CD (WDA); 1.5% beta-CD (WDB); or 1.5% oligofructose-enriched inulin (WDI). Although plasma lipids were similar after 11 weeks on theWDvs. WDA diets, aortic atherosclerotic lesions were 65% less in mice on WDA compared toWD (P < 0.05), and similar tomice fed the LFD. No effect on atherosclerosis was observed for the other WD supplemented diets. By RNA-seq analysis of 16S rRNA, addition of alpha-CD to the WD resulted in significantly decreased cecal bacterial counts in genera Clostridium and Turicibacterium, and significantly increased Dehalobacteriaceae. At family level, Comamonadaceae significantly increased and Peptostreptococcaceae showed a negative trend. Several of these bacterial count changes correlated negatively with % atherosclerotic lesion and were associated with increased cecum weight and decreased plasma cholesterol levels. Conclusion: Addition of alpha-CD to the diet of apoE-knockoutmice decreases atherosclerosis and is associated with changes in the gut flora.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Toshihiro Sakurai, Akiko Sakurai, Ye Chen, Boris L. Vaisman, Marcelo J. Amar, Milton Pryor, Seth G. Thacker, Xue Zhang, Xujing Wang, Yubo Zhang, Jun Zhu, Zhi-Hong Yang, Lita A. Freeman, Alan T. Remaley (2017), Dietary alpha-cyclodextrin reduces atherosclerosis and modifies gut flora in apolipoprotein E-deficient mice. Molecular nutrition & food research, 61(8): 1600804., which has been published in final form at 10.1002/mnfr.201600804. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

1613-4125