Oral administration of conditioned medium obtained from mesenchymal stem cell culture prevents subsequent stricture formation after esophageal submucosal dissection in pigs

Mizushima, Takeshi; Ohnishi, Shunsuke; Hosono, Hidetaka; Yamahara, Kenichi; Tsuda, Momoko; Shimizu, Yuichi; Kato, Mototsugu; Asaka, Masahiro; Sakamoto, Naoya

Oral administration of conditioned medium obtained from mesenchymal stem cell culture prevents subsequent stricture formation after esophageal submucosal dissection in pigs

Mizushima, Takeshi; Ohnishi, Shunsuke; Hosono, Hidetaka et al.

Gastrointestinal endoscopy, 2017, 86(3), 542-552

Permalink : https://hdl.handle.net/2115/71410

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アクセス権	open access
URI	https://hdl.handle.net/2115/71410
タイトル	Oral administration of conditioned medium obtained from mesenchymal stem cell culture prevents subsequent stricture formation after esophageal submucosal dissection in pigs
著者	Mizushima, Takeshi ; Ohnishi, Shunsuke ; Hosono, Hidetaka ; Yamahara, Kenichi ; Tsuda, Momoko ; Shimizu, Yuichi ; Kato, Mototsugu ; Asaka, Masahiro ; Sakamoto, Naoya
著者名 Mizushima, Takeshi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Hosono, Hidetaka
e-Rad_Researcher 50450888 著者名 Yamahara, Kenichi
著者名 Tsuda, Momoko
e-Rad_Researcher 90333608 著者名 Shimizu, Yuichi
e-Rad_Researcher 60271673 著者名 Kato, Mototsugu
e-Rad_Researcher 10113507 著者名 Asaka, Masahiro
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Endoscopic submucosal dissection
	Esophageal stricture
	Mesenchymal stem cells
	Conditioned medium
	Amnion
発行日	2017-09
出版者	Elsevier
収録物名	Gastrointestinal endoscopy
巻(号)	86(3)
ページ	542-552
抄録	Background and Aims: Endoscopic submucosal dissection (ESD) for esophageal cancer often causes postoperative stricture when more than three fourths of the circumference of the esophagus is dissected. Mesenchymal stem cells are a valuable cell source in regenerative medicine, and conditioned medium (CM) obtained from mesenchymal stem cells reportedly inhibits inflammation. In this study we evaluated whether CM could prevent esophageal stricture after ESD. Methods: We resected a semi-circumference of pig esophagus by ESD. We prepared CM gel by mixing with 5% carboxymethyl cellulose and endoscopically applied it onto the wound bed immediately after ESD and on days 8 and 15 (weekly CM group) or administered it orally from days 1 to 4 (daily CM group). We also injected triamcinolone acetonide into the remaining submucosa immediately after ESD (steroid group). We killed the pigs on day 8 or day 22 to measure the stricture rate and to perform histologic analysis. Results: Stricture rate in weekly and daily CM groups and steroid groups were significantly lower than in the control group on day 22. Moreover, CM significantly attenuated the number of activated myofibroblasts and fiber thickness on day 22. CM also significantly decreased the infiltration of neutrophils and macrophages compared with the control group on day 8. Conclusions: CM gel prevents esophageal stricture formation by suppressing myofibroblast activation and fibrosis after the infiltration of neutrophils and macrophages. Oral administration of CM gel is a promising treatment for the prevention of post-ESD stricture.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0016-5107
関連情報	PMID 28153569
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.gie.2017.01.024